Browsing by Author "Gnanadevi, R"
Now showing 1 - 8 of 8
Results Per Page
Sort Options
ArticleItem Open Access Assessment of Internalisation of Iron in the MIRB Labelled Ovine Bone Marrow Mesenchymal Stem Cells(2016-06) Gnanadevi, R; Ramesh, Geetha; Kannan, TA, et al.,; TANUVASPlates of ovine Bone Marrow Derived Mesenchymal Stem Cells(BMMSCs) were used from passage 4 to passage 6 for the present study. Internalized MIRB was observed after 72 hours of incubation in the cultured BMMSCs. The MIRB labelled cells showed decreased intensity when subcultured. The quantity of iron per cell was found to be 16.5 to 18.1 pg in BMMSCs.ArticleItem Open Access Axial Skeleton of Homing Pigeon (Columba livia)(2022) Iniyah, K; Kumaravel, A; Gnanadevi, R; TANUVASThe uniqueness of a bird includes its ability to fly, high metabolic rate and beak without teeth. The bones of birds are called as pneumatic bones since they are filled with air spaces. The pigeons are small billed birds with long wings and powerful flight musculature. They are strong and swift fliers and comes under Columbidae. The axial skeleton includes skull, vertebral column, ribs and sternum. The study is intended to provide a complete osteological profile of axial skeleton of Homing pigeon which supplement the anatomical data on pigeon and also to differentiate pigeon skeletal framework from other avian species.ArticleItem Open Access Comparative Histoarchitectural Study of Splenic Components in Sheep and Goat(Excellent Publishers, 2019) Gnanadevi, R; Senthilkumar, S; Kannan, TA; Geetha, Ramesh; TANUVASAnimal husbandry practices like sheep and goat rearing offer many advantages for beginners and also experienced farmers. They are well suited and productive for the prevailing agro-climatic conditions in India. In mammals, health status is governed by the organ of lymphatic system. Though spleen is the secondary lymphatic organ, it is the largest among the lymphoid organs which plays a crucial role in immune function. For the present study, splenic tissue from adult sheep and goat (6 Nos. each) were collected from Corporation slaughter house, Chennai. Gross, microscopic and immunohistochemical observations were done. Micrometric observations of various structures were also recorded. The spleen of sheep was triangular, whereas, in goat it was roughly quadrangular in outline. In both the species, parenchyma was covered by a thick capsule, predominantly made up of collagen, elastic and smooth muscle fibres. Thickness of capsule in sheep was 150±10.33μ and in goat it was 141±11.5μ. Trabeculae originated from inner side of the capsule and extended in to parenchyma in both the species. Parenchyma was made up of white pulp and red pulp and the proportion of these were almost equal. The white pulp was composed of splenic nodule and peri-arterial lymphatic sheath. Red pulp was compost of irregular splenic cords separated by splenic sinusoids. Immunohistochemical localization of T-lymphocytes revealed the distribution of T-lymphocytes around the PALS and also scattered in the red pulp.PresentationItem Open Access Demonstration of primary ossification centres in embryos of Aseel chicken(2020-02) Senthilkumar, S; Kannan, TA; Gnanadevi, R; Ramesh, Geetha; Hemavathi, N; TANUVASAseel chickenembryos of day five to twenty one were collected by breaking embryonated eggs purchased from Poultry Research Station, Madhavaram. Embryos were inspected for the anomalies and their morphology was studied. Embryos without abnormalities were preserved in Kaiserling preservation solution and ninth day embryo was chosen for demonstrating the primary ossification centres using alizarin red ‘S’ method. Tibitarsus, tarso-metatarsus and facial bones was the first one toossified. Epiphysis and metaphysis regions of long bones were not stained by alizarin red ‘S’ stain. Crown rump lengths (CRL) of all embryos were measured using a thread and measuring scale. Results were used in future for developmental studies of Aseel chicken breed.ArticleItem Open Access Effect of Moldey Ion Rhodamine B on Viability of Adipose and Bone Marrow Derived Mesenchymal Stem Cells - An In-vitro Study(2018-08) Gnanadevi, R; Ramesh, Geetha; Kannan, TA; Justin William, B; TANUVASThe study was performed to access the effect of Moldey ion Rhodamine B on In-vitro labelled bone marrow and adipose derived derived stem cells in sheep. Perirenal adipose tissue and bone marrow from femur were collected from 28 male sheep. Adipose derived mesenchymal stem cells were isolated from perirenal fat using enzymatic digestion method by using type II collagenase. Bone marrow derived mesenchymal stem cells were separated from femur by density gradient centrifugation method using percoll. These mesenchymal stem cells from bone marrow and adipose tissue were cultured and expanded using Dulbecco’s Modified Eagle’s Medium with 10 per-cent of fetal bovine serum, one per-cent of antibioticantimycotic solution. Adipose and bone marrow derived mesenchymal stem cells from passage 4 to passage 6 were used for labelling with medium containing 25 μg of MIRB per ml. Cell viability was evaluated by Trypan blue exclusion test in both labelled and control (unlabelled) groups. There was no significant difference in the viability of bone marrow and adipose derived mesenchymal stem cells between the labelled and control groups. It was observed that in-vitro labelling of mesenchymal stem cells with MIRB at a concentration of 25 μg per ml of medium did not affect cell proliferation and viability.ArticleItem Open Access Gross morphometric findings of udder and teat in madras red ewes(2020-03) Senthilkumar, S; Kannan, TA; Gnanadevi, R; Ramesh, Geetha; Balan, C; TANUVASThe objective of the present study was to assess the morphology and morphometric measurements in lactating and non-lactating (n=15 each) Madras Red ewes. In both the groups, udder was located in the inguinal region. It consisted of two mammary glands (right and left halves) divided by an intermammary groove and each had a single teat. In Madras Red ewes, udder circumference (UC) and inter-teat distance (ITD) showed statistically significant difference between lactating and non-lactating ewes. Udder length (UL), width of right (R-UW) and left (L-UW) quarter, udder thickness (UT), Right and left teat length (TL), teat diameter at base (TDB), teat diameter at tip (TDT), teat to floor distance (TFD), teat end to floor distance (TEFD) does not shown any statistical difference between lactating and non-lactating animals.ArticleItem Open Access GROSS MORPHOMETRIC MEASUREMENTS OF UDDER IN TELLICHERRY GOATS(TANUVAS, 2020-07) Senthilkumar, S; Gnanadevi, R; Kannan, TA, et al.,; TANUVASThe present study was conducted with the aim of establishing the basic data about gross morphometric measurements in lactating and non~lactating (n=15 each) adult Tellicherry she~goats. These goats had two mammary glands (right and left halves) divided by an inter mammary groove and each had a single teat.ArticleItem Open Access Ultrasonographic Evaluation of Mammary Gland in Boer Local Goats(2020-07) Senthilkumar, S; Kannan, TA; Ramesh, Geetha; Sumathi, D; Gnanadevi, R; TANUVASTwo-dimensional ultrasonographic examination of the udder and teats were performed in lactating and non-lactating Boer local she-goats (n=6 each) using 7.5 to 12.5 MHz linear transducer in a transverse and longitudinal plane. The udder was examined by the direct contact method. In, both the groups, the mammary gland parenchyma was uniformly echogenic. However, in lactating animals, the parenchyma showed an increased anechoic field due to accumulation of milk and increased vascularity of blood vessels. Right and left teats were examined by the water bath technique. In teat, three different layers were observed viz., anechoic teat cistern, hyperechoic teat skin, moderately echogenic middle layer and hyperechoic inner teat mucosa. Furstenberg's rosette region appeared as round, homogenous hypoechoic to anechoic. Teat orifice was hyperechoic, teat canal observed as a thin hyperechoic line between two thicker parallel hypoechoic bands. Between lactating and non-lactating animals, statistically no significant difference was observed in teat canal length (TCL), teat canal width (TCW), teat cistern diameter (TCD), teat wall thickness (TWT) and in diameter at Furstenberg’s rosette (DFR).