Browsing by Author "Gill, Jaspreet Kaur"
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ThesisItem Open Access Expression profiling of hsf1 gene and its association with oxidative stress in zebu and crossbred cattle(Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, 2015) Gill, Jaspreet Kaur; Arora, Jaspreet SinghLymphocytes are considered to be the best model to evaluate the immunity in any living body as it contains plethora of White blood cells (WBCs) .Heat stress has been an important domain of research in livestock due to its negative impact on production and disease resistance. The augmentation of stress in the body stimulate the anti oxidative activity comprising various enzymes, vitamins, minerals etc to combat the situation.The major key players involved in regulation of heat shock response in eukaryotes are the Heat Shock Factors (HSFs) which are the transcription factors. They activate the Heat Shock Protein (HSP) genes by binding to their promoters.The present research aims to determine the association of expression profile of HSF1 gene and biochemical parameters of some anti- oxidant enzymes in the peripheral blood mononuclear cells (PBMCs) in non lactating Sahiwal vis-à-vis Crossbred (Holstein freishen x Sahiwal) cattle. The PBMCs were subjected to heat shock at 39, 41 and 43°C in three different incubators, in vitro. The cell count and viability test indicate that the Crossbred are found to be more prone to heat stress as compared to Sahiwal. The qRT PCR expression data reveal in increment in HSF1 expression at 41°C subsequently decline at 43°C in both breeds post 1 hour heat shock. The enzyme assay i.e. Superoxide dismutase (SOD), Catalase, Glutathione Reductase (GSH) and Malonidialdehyde (MDA) also showed changes in their magnitude following exposure to heat stress. However, the correlation between the biochemical test parameters and the HSF1 expression were mostly non-significant (P<0.05). Breed-wise analysis of the predictability of the HSF1 expression through these enzymatic assays were also poor (R2<40%). This suggests that expression of HSF1 gene is independent of the enzymatic activity in vitro in zebu and crossbred cattleThesisItem Restricted Simultaneous Grafting and Rooting Studies in peach [Prunus persica (L.) Batsch](Punjab Agricultural University, 2014) Gill, Jaspreet Kaur; Harminder SinghPresent investigations on the “Simultaneous Grafting and Rooting Studies in peach [Prunus persica (L.) Batsch]” was carried out at Fruit Research Farm, Department of Fruit Science, Punjab Agricultural University, Ludhiana during the year 2013-14. 15-20 cm long hardwood cuttings of peach rootstock Flordaguard were taken from 6 year old trees on 5th and 15th January, 2013. These cuttings were tongue grafted with scions of Earli Grande and Shan-i- Punjab having 3-4 buds on them. After grafting, the 3/4 cm basal portion of the grafts were treated with different concentrations of IBA treatments viz. @ 0, 100, 200 (24 hr soaking), 1000, 1500, 2000, 2500 and 3000 ppm (2 minutes dip) and were planted in the field. Sprouting in both the peach cultivars in all the treatments started in the 1st week and completed in the 2nd week of February. Sprouting success was found to be highest in 2000 ppm IBA treatment. The vegetative growth (plant height, stem thickness and number of leaves) of the graft was also found to be maximum in 2000 ppm IBA. Highest mortality of the grafts in all the treatments were recorded in May and after that no/ little mortality was observed. Mortality rate was found to be minimum in higher doses of IBA and maximum in control and lower doses of IBA treatment. The root length and number of roots of both Shani- Punjab and Earli Grande grafts was found to be higher in 2000 ppm IBA treatment. The sprouting success and vegetative growth of all the grafts in all the treatment were better when the cuttings were made on 15th January than on 5th January. The number of salable plants was also observed to be maximum in 2000 ppm IBA treatment in both the cultivars of peach