Browsing by Author "Dillibabu, V."
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ArticleItem Open Access Genetic diversity of different avian strains of Pasteurella multocida recovered during four outbreaks in India(2018-02) Karthik, K.; Dillibabu, V.; Mahaprabhu, R.; Bharathi, R.; Manimaran, K.; Shoba, K.; TANUVASPasteurella multocida causes fowl cholera in avian species and type A is predominantly reported from outbreaks of fowl cholera. Biochemical, serological, and molecular methods are employed for its diagnosis and typing. Utilizing rapid molecular tools like repetitive extragenic palindromic (REP) PCR and enterobacterial repetitive intergenic consensus (ERIC) PCR, P. multocida isolates from four outbreaks (three from Chennai and one from Ahmadabad, India) were characterized and typed to determine their relationships. A total of 36 isolates were recovered from the outbreaks, including one isolate from a parakeet, which was also subjected to characterization by conventional and molecular analysis. All of the isolates were found to be capsular type A based on PCR assay capsular typing. ERICand REP-PCR showed differences in the banding patterns among different outbreak isolates, and also among the geographical regions. Differences were also noticed among different host species, as the banding pattern in the ERIC- and REP-PCR differed; the analysis of results also revealed the same. All of the isolates were found to be sensitive to enrofloxacin and cefotaxime among the antibiotics used in the study. It was found that different strains might have been involved in the different outbreaks reported in the study. The results show that molecular typing methods like ERIC- and REP-PCR are useful epidemiological tools for classifying the strains.ArticleItem Open Access Hepatic Abscess in Sheep and Goat Caused by O26 Escherichia Coli Serotype : An Emerging Pathogen(2015-07) Madhav, N. Mugale; Balachandran, C.; Dillibabu, V.; Kirubharan, John; Raj, G. Dhinakar; Sridhar, R.; Selvasubramaniam, S.; TANUVASLiver abscess is a major economic problem in meat industry due to condemnation of edible part of carcass. In this study, an incidence 0.224% (56/25000) of hepatic abscess was recorded. Escherichia coli was isolated in highest number of cases which showed metallic sheen on eosin methylene blue agar in 12 cases (21.48%). Serotype O26 E. coli was identified and recognized as an emerging pathogen.ArticleItem Open Access Hepatic Abscess in Sheep and Goat Caused by O26 Escherichia Coli Serotype : An Emerging Pathogen(Indian Veterinary Journal, 2015-07) Madhav, N. Mugale; Balachandran, C.; Dillibabu, V.; Kirubaharan, John; Raj, G. Dhinakar; Sridhar, R.; Selvasubramaniam, S.; TANUVASLiver abscess is a major economic problem in meat industry due to condemnation of edible part of carcass. In this study, an incidence 0.224% (56/25000) of hepatic abscess was recorded. Escherichia coli was isolated in highest number of cases which showed metallic sheen on eosin methylene blue agar in 12 cases (21.48%). Serotype O26 E. coli was identified and recognized as an emerging pathogen. Other pathogens isolated were Streptococcus spp., Fusobacterium necrophorum, Staphylococcus spp., Corynebacteria spp. and Pseudomonas spp. Grossly, single to multiple and minute to large creamy-yellow coloured abscesses of varying sizes were found on both parietal and visceral surfaces of liver. Histologically, the abscesses consisted of central areas of liquefactive necrosis surrounded by numerous polymorphonuclear neutrophils, few mononuclear cells, calcified centers and colonies of bacteria.ArticleItem Open Access Isolation and Identification of Bacteria of Normal Flora in Handfed Exotic Psittacine Birds at Tamil Nadu, India(TANUVAS, Chennai, 2019-03) Legadevi, R.; Dillibabu, V.; Karunakaran, N.; Nagarajan, K.; Soundararajan, C.; TANUVASBacterial normal flora were identified in handfed psittacine birds (lory, amazons, lorikeet, cockatoo. conure, grey parrot, macaw, eclectus and African love birds) at Cavin estate exotic bird farm, Chennai. Tamil Nadu. A total of 54 cloacal swabs were collected from different species of psittasine birds for bacteriological screening to identify the microorganisms by morphological and biochemical characteristics.ArticleItem Open Access Isolation of Bacteria From Dead - In - Shell Chick Embryos of Psittacines(TANUVAS, Chennai, 2019-03) Dillibabu, V.; Legadevi, R.; Karunakaran, N.; Nagarajan, K.; Tamilmani, S.; Kaushika, S.; Jayakeerthil, S.; Soundararajan, C.; TANUVASThe objective of the present study is to explore the bacteria associated with the dead- in-shell eggs of psittacines. A total of 132 dead-in-shell eggs of different psittacine breeds (Macaws, Lories, Conures. Amazons, Eclectus, Cockatoos, Grey parrots and Jardine parrots) were obtained from the incubation unit of Cavin estate exotic bird farm, Chennai, Tamil Nadu and subjected to microbological analysis. The overall prevalence of bacteria isolated from the dead-in-shell was 80.30% (l06/ 132). Among these, Gram-negative bacteria were predominant (49.24%, 65/132) was observed followed by Gram-positive organisms (28.78%, 38/132) and Gram-positive filamentous organisms (2.27%, 3/132) were also isolated from dead in shell embryos.