Browsing by Author "DOLEY, PROBAL JYOTI"
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ThesisItem Open Access COMPARATIVE CYTOMORPHOLOGICAL, CYTOCHEMICAL, CYTOENZYMIC AND ULTRASTRUCTURAL STUDIES ON THE BLOOD CELLS OF ADULT RHODE ISLAND RED, ASEEL AND NON DESCRIPT INDIGENOUS CHICKEN OF MIZORAM(College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, 2021-12) DOLEY, PROBAL JYOTI; Sarma, KabitaMatured erythrocytes were elliptical in shape and their mean length was measured to be 12.65±0.12 μ, 12.62±0.19 μ and 12.52±0.10 μ and their mean width was measured to be 6.83±0.09 μ, 6.81±0.11 μ and 6.07±0.07 μ in Rhode Island Red, Aseel and Zoar respectively. The hetrophils were the largest granulocyte and had a mean diameter of 10.01±0.16 μ, 10.23±0.12 μ and 9.98±0.23 μ while the basophils were the smallest granulocyte and had a mean diameter of 8.41±0.17 μ, 8.37±0.14 μ and 8.24±0.18 μ in Rhode Island Red, Assel and Zoar respectively. The eosinophils were almost equal to the heterophils and measured 9.53±0.10 μ in Rhode Island Red, 9.61±0.10 μ in Aseel and 9.82±0.24 μ Zoar. The monocytes were the largest leukocyte and had a mean diameter of 11.35±0.15 μ in Rhode Island Red, 11.97±0.27 μ in Assel and 11.43±0.10 μ in Zoar. The lymphocytes were of variable sizes and their mean diameter ranged from 5.96±0.13 μ, 6.09±0.11 μ and 5.67±0.13 μ to 9.35±0.48 μ, 9.88±0.20 μ and 9.13±0.04 μ in Rhode Island Red, Assel and Zoar respectively. The thrombocytes were the smallest blood cell and had a mean diameter of 4.85±0.10 μ, 4.93±0.15 μ and 4.47±0.08 μ in Rhode Island Red, Aseel and Zoar respectively. Reticulocytes and siderocytes were rare in the blood of Rhode Island Red, Aseel and Zoar. The heterophils of Rhode Island Red, Aseel and Zoar were positive for SBB, PAS, acid phosphatase, alkaline phosphatase and arylsulphatase while the eosinophils were positive for SBB, PAS, alkaline phosphatase, cytochrome oxidase and peroxidase. The basophils of Rhode Island Red, Aseel and Zoar were positive for toluidine blue while the thrombocytes were positive for PAS. Under Scanning Electron Microscopy the erythrocytes appeared elliptical in shape while the leukocytes and thrombocytes appeared round in shape with variable surface modifications. Under Transmission Electron Microscopy the granules of the heterophils of Rhode Island Red, Aseel and Zoar appeared predominantly fusiform in shape, the granules of the eosinophils appeared round in shape and that of the basophils appeared pleomorphic in shape. The cytoplasm of the monocytes, medium to large lymphocytes and thrombocytes of Rhode Island Red, Aseel and Zoar under Transmission Electron Microscopy appeared to be vacuolated and granular while that of the small lymphocytes appeared to be non vacuolated and granular.