Browsing by Author "Brindha, K."
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OtherItem Open Access Combined Inactivated Vaccine Against Newcastle Disease and Fowl Cholera(TANUVAS, Chennai, 2007-08) Brindha, K.; Ronald, B.S.M.; Chandran, N. Daniel Joy; Ravikumar, G.; Dhiagarajan; Jeyachitra, V.; Kumar, N. Krishna; Manohar, B. MuraliOtherItem Open Access Comparison of DNA Extraction Methods From Bovine Semen for Detection of Bovine Herpes Virus Type-1 by Polymerase Chain Reacion(TANUVAS, Chennai, 2012-09) Brindha, K.; Ravikumar, G.; Chandran, N. Daniel Joy; TANUVASOtherItem Open Access Comparison of Various Methods of DNA Extraction From Liver Samples for the Detection of Hydropericardium Syndrome Virus by Polymerase Chain Reaction(TANUVAS, Chennai, 2007-08) Brindha, K.; Sriram, T.K.; Ravikumar, G.; Chandran, N. Daniel Joy; Koteeswaran, A.ArticleItem Open Access Comparison of Virus Isolation and Polymerase Chain Reaction for Diagnosis of Peste Des Petits Ruminants(Acta Virologica, 2001) Brindha, K.; Raj, G. Dhinakar; Ganesan, P.I.; Thiagarajan, V.; Nainar, A.M.; Nachimuthu, K.; TANUVASOculonasal swabs and tissue samples collected from peste des petits ruminants (PPR) suspected sheep and goats were tested for presence of the virus of peste des petits, ruminants (PPRV) or its RNA by reverse transcription-PCR (RT-PCR) and virus isolation (VI). Of 44 samples 31.8% and 40.9% were positive by VI and RT-PCR, respectively. The RT-PCR-positive samples were subjected to the nested PCR. Three of six samples positive by RT-PCR but negative by VI were negative by the nested PCR. The specificity and accuracy of the nested PCR were higher than those of the RT-PCR although the sensitivity of both tests were similar. Nucleotide sequencing of one nested PCR product revealed a 92% homology with the sequence available in the GenBank (Acc. No. Z37017).ArticleItem Open Access DETECTION OF SINGLE NUCLEOTIDE POLYMORPHISM OF A1/A2 VARIANTS OF BETA CASEIN GENE IN UMBALACHERY CATTLE BY TETRA ARMS PCR(TANUVAS, Chennai, 2018-05) Nila, R. Kalai; Brindha, K.; Reddy, Y. Krishnamohan; Baskaran, D.; TANUVASAll over the world, people fulfill 13% of their protein requirements through milk and milk products (Cifelli et al., 2015). Bovine milk contains two major protein groups namely caseins and whey proteins (Bell et al., 2006).ThesisItem Open Access DEVELOPMENT OF SPRAY DRIED MILK POWDER E USING MILK FROM A2 GENOTYPED NATIVE CATTLE(TANUVAS, Chennai, 2017) Kalainila, R.; Brindha, K.; Reddy, Y. Krishnamohan; Gnanalakshmi, K.S.; TANUVASThe present research work was carried with the objective of identifying A1 and A2 variants of beta casein gene in umbalachery breed and to develop spray dried milk powder from A2 milk. Bovine genomic DNA was extracted using Phenol: Chloroform: lso amyl alcohol method. Tetra Amplification Refractrory Mutation System Polymerase Chain Reaction was used to analysis A1 and A2 beta casein variants in umbalachery cattle.OtherItem Open Access Doppler Pulse Frequency of Peri-Follicular Blood Flow: An Indicator of Ovulation in Crossbred Cows(TANUVAS, Chennai, 2013-06) Satheskumar, S.; Asokan, S.A.; Brindha, K.; Kathiresan, D.; Kumanan, K.ArticleItem Open Access Effect of Cysteamine Supplementation in SOF Medium on in vitro Culture of Buffalo Embryos(Indian Veterinary Journal, 2011-08) Indira, R. Lilly; Palanisamy, A.; Meenambigai, T.V.; Ramaswami, V.; Brindha, K.; Kumanan, K.; TANUVASIn the present study, oocytes were collected from slaughter house ovaries, sliced, in vitro matured and fertilized with buffalo sperms processed by swim up technique. The presumptive zygotes were culture in SOF medium in control group and SOF with cysteamine in treatment group.ArticleItem Open Access Effect of Follicular Fluid Supplementation On In-Vitro Maturation And Developmental Rate Of Buffalo Oocytes(2017) Satheshkumar, S.; Priya, M. Shalini; Brindha, K.; Sakthivel, S.; Kumanan, K.; TANUVASThe effect of supplementation of follicular fluid (FF) at 5.0% concentration in in vitro maturation (IVM) media on in vitrn production of buffalo embryos was studied. Oocytes were aspirated from slaughter house derived ovaries and graded.ArticleItem Open Access EFFECT OF FOLLICULAR FLUID SUPPLEMENTATION ON IN-VITRO MATURATION AND DEVELOPMENTAL RATE OF BUFFALO OOCYTES(West Bengal Veterinary Association, 2017-06) Satheshkumar, S.; Priya, M. Shalini; Brindha, K.; Sakthivel, S.; Kumanan, K.; TANUVASThe effect of supplementation of follicular fluid (FF) at 5.0% concentration in in vitro maturation (IVM) media on in vitro production of buffalo embryos was studied. Oocytes were aspirated from slaughter house derived ovaries and graded. Cultutarable quality oocytes were randomly cultured in IVM media with FF (n = 334) and without FF (n = 228) and subjected to maturation. The maturation rate, cleavage rate and embryo developmental rate was recorded in both the groups. The maturation, cleavage, morula and blastocyst rates (78.2 ± 1.8, 41.2 ± 1.3, 15.2 ± 0.7 and 7.7 ± 0.9 % respectively) were significantly (P < 0.01) higher when oocytes were matured in media supplemeted with FF than controls (55.3 ± 1.9, 28.7 ± 1.5, 6.7 ± 0.8 and 2.2 ± 0.8 % respectively). Thus, it could be concluded that supplementation of FF (5.0 %) to IVM media would benefit the maturation, cleavage and embryo development in buffalo IVF programme.ArticleItem Open Access Effect of Physico-Biochemical Characteristics of Follicles on Quality and In Vitro Maturation of Bubaline Oocytes(2016) Satheshkumar, S.; Revathipriya, B.; Brindha, K.; Roy, A.; Kumanan, K.; TANUVASThe physico-biochemical properties of follicles and its plausible effect on quality and maturational competency of oocytes during the different breeding periods of the year was studied in buffaloes (Bubalus bubalis). Ovaries were collected from sexually mature buffaloes slaughtered at abattoir during high breeding (December- February; HB) and low breeding (April-July; LB) periods of the year. The surface antral follicles were categorized as small (SF; < 4 mm), medium (MF; 4-9 mm) and large (LF; >9 mm) follicles. The follicular fluid (FF) was aspirated from follicles of different categories and the recovered oocytes were assessed for their quality. The good quality oocytes were cultured in vitro to assess the maturational competency. The glucose (Glu), total proteins (TP) and triglycerides (Tgl) concentrations of FF were analysed and correlated with the oocyte quality and maturation capacity. Among the follicular categories, significantly (P<0.01) more percentage of good quality oocytes with high maturation rate was recovered from MFs and vice versa for oocytes from LFs in both the periods. The mean percentage of good quality oocytes and the mean percentage of oocytes that reached the MII stage was significantly (P<0.01) higher during HB than LB period. The Glu concentration increased as the follicle size increased, while the TP concentration remains similar in all follicular categories but the Tgl concentrations were significantly higher in SFs. In general, the concentrations of these three biochemical components, with significance to Tgl, were found to be increased in the FF during the LB period correlating with the deteriorated oocyte quality and developmental properties. This might mean that metabolic activities were less intensive resulting in lower consumption of these components by the follicular cells and oocytes during the LB than HB period leading to fall in fertility levels during the former period.ThesisItem Open Access Embryotrophic Potential Of Novel And Candidate Oviductal Fluid Proteins Of Buffalo(Tamil Nadu Veterinary and Animal Sciences University, 2014) Brindha, K.; TANUVAS; Parthiban, M.; Chandran, N. Daniel Joy; Raj, G. Dhinakar; Balasubramanian, S.The mammalian oviduct is the site of fertilization and early cleavage providing an ideal milieu for early embryonic development. The physiological interaction between gametes or embryos and the oviducts involves intimate and precise signalling to establish and maintain a healthy conceptus. The present study was undertaken to investigate the alterations in the oviductal proteins that arise due to cyclical changes and upon co-incubation of gametes which inturn would lead to identification of putative embryotrophic factors that could be used to improve the outcome of in vitro embryo production. Oviductal epithelial cells (OEC) derived from early, mid and late phase of the oestrous cycle were used for quantitative RT-PCR and oviductal fluid for protein analysis by 2D gel electrophoresis. Expression profiling of candidate genes namely Heat shock protein 70 (Hsp70), Oviduct specific glycoprotein (OVGP) and Osteopontin (OPN) revealed that the transcripts were differentially regulated by hormonal influence. Expression of OVGP and OPN mRNA was found to be maximal during the early phase of the cycle. However, expression of Hsp 70 was not found to differ much between the different phases of the oestrous cycle. Co-incubation of OEC with oocytes, sperms and both the gametes resulted in upregulation of Hsp 70, OVGP and OPN genes when compared to control. However the upregulation was maximum upto 36-fold for OVGP gene when OEC was co-incubated with both the gametes instead of either of them. One of the candidate proteins, Hsp 70, upon supplementation to the embryo culture medium was found to increase the number of expanded COCs and viable oocytes to 84.21 ± 1.14 % and 87.77 ± 2.42% when compared to the 74.34 ± 1.50% and (76.38 ± 2.75%) in the control group. Osmotolerance, acrosome reaction, viability and sperm-binding rate of Hsp 70 supplemented spermatozoa were found to be better than the control. The yield of presumptive zygotes (52.34±0.78%) and morula (36.73±1.07%) were higher in Hsp 70 supplemented group than in the control (37.71±2.18% and 26.36±2.0%). Analysis of the oviductal fluid proteins by 2D gel revealed upregulation of 29 proteins and downregulation of 23 proteins in the gamete treated group. A total of 17 differentially expressed proteins were identified by MALDI-TOF/MS out of which two novel proteins namely thioredoxin and serine threonine protein phosphatase (PP2A) were analyzed for their functional role by supplementation in in vitro embryo culture medium. Results of the supplementation studies revealed that both these oviduct-derived proteins possessed embryotrophic properties.ArticleItem Open Access Establishment of Embryonic Stem Cell like Cell Colonies from In-vitro Produced Buffalo Compact Morulae(TANUVAS, Chennai, 2019-05) Satheshkumar, S.; Brindha, K.; ShaliniPriya, M.; Parthiban, M.; Palanisammi, A.; Raj, G. Dhinakar; TANUVASThe study was aimed at establishing the embryonic stem cells (ESC) like cell colonies from in-vitro produced buflalo (Bubalus bubalis) compact morula (CM). A total of 33 CM were subjected for zonalysis using pronase and embryonic cells were derived by three different methods viz., T 1: Intact CM; T 2: Disaggregation of CM by gentle pipetting and T 3: Slicing of CM using Bard Parker blade.ArticleItem Open Access Expression of GAP Junctional Protein - Connexin 43 in Buffalo Cumulus Occyte Complexes(Indian Veterinary Journal, 2009-09) Meenambigai, T.V.; Veenavardhini, S. Vishnu; Palaniswamy, A.; Brindha, K.; Satheshkumar, S.; Rangasamy, S.; Kumanan, K.; TANUVASArticleItem Open Access Expression Of Pregnancy Associated Glycoproteins (PAGs) During Different Stages Of Pregnancy - A Marker For Early Pregnancy Diagnosis In Buffaloes(2015-12) Rangasamy, S.; Balasubramanian, S.; Vadivoo, V.S.; Brindha, K.; Gopikrishnan, D.; Kulasekar, K.; TANUVASOtherItem Open Access Expression Pattern of Pregnancy Associated Glycoproteins (PAG) During Different Stages of Pregnancy - A Marker for Early Pregnancy Diagnosis in Buffaloes(TANUVAS, 2014-12) Rangasamy, S.; Balasubramanian, S.; Vadivoo, V.S.; Brindha, K.; Kulasekar, K.ArticleItem Open Access Full-Genome Sequence Analysis of a Reassortant Strain of Bluetongue virus Serotype 16 from Southern India(2016-08) Kumar, Lalit; Batra, Kanisht; Chaudhary, Deepika; Brindha, K.; TANUVASOtherItem Open Access Identification of Thioredoxin as an Embryotrophic Molecule involved in the Maternal Embryo Communication in Buffalo(TANUVAS, 2014-12) Brindha, K.; Parthiban, M.; Raj, G. Dhinakar; Chandran, N. Daniel Joy; Balasubramanian, S.