Browsing by Author "Baswaraj.R."
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ThesisItem Open Access Characterization, serodiagnosis and management of virus associated with bud blight of soybean in karnataka(UAS Dharwad, 2010) Baswaraj.R.; A.S.ByadgiSoybean bud blight, an important viral disease was studied with an objective of biological and molecular characterization and management including screening for resistant lines. Roving survey conducted in Karnataka during 2007-09 showed minimum incidence during Kharif (1.6%; 2007) in Dharwad district and maximum during Rabi (20.2%; 2008-09) in Belgaum district. Under field conditions, virus produced downward curving of terminal bud showing hook like appearance. Virus was partially purified and confirmed by electron microscopy, DAC-ELISA and by RT-PCR using TSV-CP primer which confirmed the causal virus as Tobacco Streak Virus. The CP gene was cloned and was found 398bp in length. The CP gene shared 99.7 per cent sequence homology with already reported TSV from DWD-8, GWD-2, Latur, Beed, Solapur, Raichur and Coimbatore. Hence it was designated as TSV-Sb (Sb; soybean). Sap transmission of virus was successful by 0.05 M potassium phosphate buffer 7.0 pH containing 0.1 per cent 2- Mercaptoethanol. The virus was not seed borne. Among insects only thrips could transmit the virus. TSV-Sb has thermal inactivation point of 500C, dilution end point of 1 x 10-4 and longevity in vitro was 8 hours. Other than soybean, TSV-Sb can infect Helianthus annus (L.) Arachis hypogaea (L), Glycine max (L), Vigna unguiculata (L), Chenopodium quinoa (L.), Lycopersicon esculentum (Mill) and Capsicum annuum (L.) not Cucumis melo (L.). Antiserum was produced against TSV-Sb by immunizing rabbit with partially purified virus preparation and was cross adsorbed. The purified antiserum found positive in slide agglutination, micro precipitation, immunodiffiusion tests and DAS-ELISA. The titer of the antiserum raised against TSV-Sb was 1:512. Management studies showed border cropping with sorghum plus imidacloprid (@ 0.25 ml/lit) spray will reduce the disease. None of the genotypes screened were immune but NRCS 7, MAUS 71 showed resistant and can be used under integrated disease management schedule.