Browsing by Author "BINDU BHARGAVI, G"
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ThesisItem Open Access EVALUATION OF OIL BASED FORMULATIONS OF Nomuraea rileyi (FARLOW) SAMSON AGAINST Spodoptera litura FABRICIUS(Acharya N.G. Ranga Agricultural University, 2018) BINDU BHARGAVI, G; MANJULA, KThe liquid formulations of Nomuraea rileyi, an important entomopathogenic fungus were prepared by using two vegetable oils and two mineral oils viz., olive oil, rice bran oil, liquid paraffin oil, heavy grade mineral oil. N. rileyi spore mass was harvested from culture plates and mixed to autoclaved test oils in the proportions of 0.1g (0.5 x 108 spores/0.1 g) and 0.2g (0.1 x 109 spores/0.2 g) per 100ml. Triton-X 100, a wetting agent was also used in two different concentrations i.e., 0.05% and 0.1% for all four test oils. Among the 16 oil based formulations of N. rileyi, rice bran oil with 0.2g spores and 0.1ml triton-X 100 oil formulation recorded highest conidial germination. It was ranged from 68-93 per cent up to 150 days. Liquid paraffin with 0.2g spores and 0.1ml triton-X 100 and heavy grade mineral oil with 0.2g spores and 0.1ml triton-X 100 oil formulations recorded 64-90 per cent and 62-89 per cent conidial germination respectively. The relatively lowest conidial germination of 35-71 per cent was observed in olive oil with 0.1g spores and 0.05 ml of triton-X 100 oil formulation. The remaining formulations recorded 35-87 per cent conidial germination. Correspondingly, rice bran oil with 0.2g spores and 0.1ml triton-X 100 oil formulation recorded highest larval mortality of 78-91 per cent followed by liquid paraffin with 0.2g spores and 0.1ml triton-X 100 and heavy grade mineral oil with 0.2g spores and 0.1ml triton- X 100 oil formulation which recorded 74-89 and 69-86 per cent respectively. The remaining formulations recorded 28-85 per cent larval mortality. Among the 16 oil based formulations of N. rileyi tested in the laboratory, based on the better performance, eight were selected for field evaluation against S. litura in Rabi groundnut. In the field also, rice bran oil with 0.2g spores and 0.1ml triton-X 100 oil formulation recorded highest per cent larval reduction of 82 per cent at 20 days after treatment. Liquid paraffin with 0.2g spores and 0.1ml triton-X 100 and heavy grade mineral oil with 0.2g spores and 0.1ml triton-X 100 oil formulations also stood as next best treatments. Least performance was observed by olive oil with 0.1g spores and 0.05 ml of triton-X 100 oil formulation (49 per cent larval reduction). The remaining formulations recorded 58-71 per cent larval reduction.