Browsing by Author "BHAVANA, S."
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ThesisItem Open Access CERTAIN STUDIES ON THE INCIDENCE AND DIAGNOSIS OF CANINE DISTEMPER IN DOGS(KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2019-09) BHAVANA, S.; Dr. H.A. UPENDRACanine Distemper (CD) caused by canine Distemper virus (CDV) is one of the most severe and infectious viral diseases of dogs and many other carnivores producing high morbidity and mortality. The present study was carried out to detect the presence CDV in suspected dogs by Immunochromatographic (IC) test and by RT-PCR. Sixty dogs were diagnosed with CDV based on clinical manifestation indicating an incidence of 0.75 per cent of which 43.33 per cent were found to be positive for CD when the clinical samples (ThesisItem Open Access CERTAIN STUDIES ON THE INCIDENCE AND DIAGNOSIS OF CANINE DISTEMPER IN DOGS(KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2019-09) BHAVANA, S.; . H.A. UPENDRACanine Distemper (CD) caused by canine Distemper virus (CDV) is one of the most severe and infectious viral diseases of dogs and many other carnivores producing high morbidity and mortality. The present study was carried out to detect the presence CDV in suspected dogs by Immunochromatographic (IC) test and by RT-PCR. Sixty dogs were diagnosed with CDV based on clinical manifestation indicating an incidence of 0.75 per cent of which 43.33 per cent were found to be positive for CD when the clinical samples (nasal/conjunctival swab) were subjected to IC test, indicating that clinical manifestation alone cannot be the criteria for diagnosis of CD. Upon haematological evaluation, Neutrophilic leukocytosis with lymphopaenia, anaemia and thrombocytopaenia wereThesisItem Open Access STUDIES ON YELLOW MOSAIC DISEASE OF MAJOR GOURDS(Keladi Shivappa Nayaka University of Agricultural & Horticultural Sciences, Shivamogga, 2021-12-18) BHAVANA, S.; Dr. R. GANESHA NAIK; Dr. H. RAVINDRAYellow mosaic disease (YMD) in ridge gourd, bitter gourd, ivy gourd and bottle gourd caused by tomato leaf curl New Delhi virus (ToLCNDV) was an economically important disease which hampered the crop growth and yield. ToLCNDV is a begomovirus that was persistently transmitted by whitefly (Bemisia tabaci). Among major diseases occurring on gourds, YMD was one of the most prevalent and serious problem in Karnataka. Roving survey was conducted during summer 2020-21 to assess the incidence of YMD in Chikmagalur, Davangere and Shivamogga districts. Survey results revealed that the highest disease incidence of 58.18 per cent in ridge gourd, 59.21 per cent in bitter gourd, 32.50 per cent in ivy gourd and 42.50 per cent in bottle gourd were documented. Among the district surveyed, Davanagere district has recorded the highest mean disease incidence of YMD in all the gourds. Suspected YMD infected samples collected during surveyed areas were tested by PCR using begomovirus specific primers for conserved region, Rep associated protein and coat protein gene. The PCR product of 560bp and 1200bp was obtained, respectively. The nucleotide sequences of replication associated protein and coat protein gene of YMD infected ridge gourd, bitter gourd, ivy gourd and bottle gourd showed 99.72, 98.94, 99.00 and 100 per cent identity with ToLCNDV, respectively. Phylogenetic analysis of Rep associated protein and coat protein gene sequences of ToLCNDV isolates of ridge gourd, bitter gourd and ivy gourd formed a single cluster, while bottle gourd formed a separate cluster. Ridge gourd yellow mosaic virus (strain of ToLCNDV) has been successfully transmitted by whiteflies (Bemisia tabaci). Inoculating 10 adult whiteflies per plant with 12h of acquisition access period (AAP) and inoculation access period (IAP) resulted in 100 per cent virus transmission. The virus transmitted in persistant manner and retained in whiteflies for 5-8 days.