Browsing by Author "Arthanarieswaran, M"
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ArticleItem Open Access Detection of Follicle Stimulating Hormone Receptor Gene Polymorphism in Murrah and Graded Murrah Buffaloes(2019-07) Kathiravan, RS; Chitra, R; Murali, N; Arthanarieswaran, M; TANUVASA study was undertaken in 203 Murrah and graded Murrah buffaloes reared under organised farm and farmers field conditions in Tamil Nadu and Andhra Pradesh to study the polymorphism of FSHR gene by PCR- RFLP method and to analyse the possible associations of these gene variants with age at first calving and calving intervals. The PCR amplification carried out with specific forward (F - 5’- CTG CCT CCC TCA AGG TGC CCC TC -3’) and reverse (R – 5’- AGT TCT TGG CTA AAT GTC TTA GGG GG -3) primers. The 306 bp amplified PCR products of FSHR (exon 10) gene was genotyped based on RFLP with AluI restriction enzymes and all the tested animals were monomorphic and showed unique banding pattern which indicated the fixation of C allele in buffaloes.ArticleItem Open Access Genetic Polymorphism of Estrogen Hormone Receptor (Exon C) Gene in Buffaloes(Excellent Publishers, 2018-02) Kathiravan, RS; Chitra, R; Murali, N; Arthanarieswaran, M; TANUVASEstrogen hormone is an important hormone found to be linked with reproductive traits and affect the growth, differentiation and function of reproductive tissues. Estrogen receptor gene (ERα) is considered as candidate marker for fertility traits in farm animals. The present study was undertaken with 203 buffaloes from different locations. The Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) of ER-α gene revealed monomorphic pattern at 171 and 77 bp and genotyped as GG for all the tested animals, which indicates the fixation of allele. As a conclusion, monomorphic pattern of Estrogen Receptor-α (ERα) gene is considered a unique feature that may be related to the characteristic of buffaloes.ArticleItem Open Access PCR BASED DETECTION OF COAGULASE GENE ENCODING STAPHYLOCOCCUS AUREUS ISOLATED FROM SUBCLINICAL MASTITIS CASES IN BUFFALOES(2010) Malmarugan, S; Arthanarieswaran, M; Suresh, P; TANUVASThe present study was carried out to characterize Staphylococcus aureus (S. aureus) isolated from mastitic cases in buffalo based on coagulase gene. A total of 18 strains of S. aureus were isolated during processing of 81 milk samples from 21 buffaloes. The S. aureus strains were characterized phenotypically, and were further characterized genotypically by polymerase chain reaction using oligonucleotide primers that amplified genes encoding coagulase (Coa). Amplification of the Coa gene yielded a product size of 730bp in all 15 coagulase positive isolates. The phenotypic and genotypic findings of the present strategies might provide an understanding of the distribution of the cogulase positive S. aureus clones among buffaloes mastitis isolates, and might aid in the development of steps to control S. aureus infections in buffaloes.ArticleItem Open Access Polymorphism in exon 13 of estrogen hormone receptor (Erα) gene by PCR-RFLP in Murrah/ graded Murrah buffaloes(2017-10) Kathiravan, RS; Chitra, R; Murali, N; Arthanarieswaran, M; TANUVASThe present study was undertaken with 204 buffaloes during June, 2016 to July, 2017 from different locations viz., Saraswathi Krishi Vigyan Kendra, Karur district, Tamil Nadu; Post Graduate Research Institute in Animal Sciences (TANUVAS), Katupakkam, Tamil Nadu; Central Cattle Breeding Farm, Alamadhi, Chennai, Tamil Nadu; Buffalo Research Station, Venkataramanna Gudem, S.V.V.U, West Godavari District, Andhra Pradesh and Farmers herd in Namakkal, Tamil Nadu to determine the genetic variation. The animals were genotyped by Polymerase Chain Reaction – Restriction Fragment Length Polymorphism (PCR-RFLP). In the PCR reaction the primers used were 5’ AGC ATC TAT ACA GCA TGA AGT G 3’ and 5’ GTG TAC TAA GCT GAC AAA GTC TTG 3’. An 870 bp long ER-α (exon 13) gene PCR product was genotyped for polymorphic pattern using MboI restriction enzymes. The PCR-RFLP of ER-α gene (exon 13) showed 614 and 256 bp long digested PCR products in all the tested samples. Monomorphic pattern as AA genotype for all the tested animals indicates the fixation of this allele in Murrah buffaloes.ProceedingsItem Open Access Response of Autogenous Vaccine at the Phase of Orf Outbreak in GoadsSuresh, P; Arthanarieswaran, M; Sukumar, K; Gunaseelan, L; TANUVASThere was an outbreak suspected to be of Contagious Eethyma (Orf) in Goats with typical proliferative and ulcerative lesions with broad base on commissures of mouth and muzzle in a hamlet located at namakkal district.ArticleItem Open Access A Retrospective Study on the Prevalence of Infectious Diseases in Desi Fowls of Tirupur District, India(2020-06) Vadivoo, VS; Arthanarieswaran, M; Lakshmikantan, U; Hariharan, T; Mathivananan, R; TANUVASThis retrospective study was undertaken to determine the prevalence of common infectious disease that caused major mortality and economic loss in desi chicken farming in Tirupur district of Tamil Nadu. Disease prevalence was studied in a total of 261 ailing or dead desi chicks that were presented for disease diagnosis to the Veterinary University Training and Research Centre, Tirupurbetween 2017 to 2019.Majority of desi birds were affected by Newcastle disease (ND) (27.59%) followed by Fowl cholera (16.86), Aspergillosis (8.42 %), Colibacillosis (7.66%), Coccidiosis (7.66%), Fowl pox (7.66 %), Heat stress (6.89 %), Worm infestation (5.36%) Respiratory infection (3.83)and Nutritional deficiency (3.06 %). Death due to Lymphoidleukosis (1.92 %), infectious bursal disease (0.77%), Mycoplasma (0.77 %), gout (0.77%) and Pecking (0.77%), was very minimum. Birds in the age group of 9-18 weeks showed high prevalence (44.06 %) of disease followed by the young chicks in the age group of 0-8 weeks (40.23 %). Adult birds more than 18 weeks of age were the least affected (15.71 %) in this study. Similarly the diseases were recorded frequently during summer (40.61%) followed by rainy (31.80%) and winter season (27.59%).