Browsing by Author "Aravindan, K."
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OtherItem Open Access Comparison of PCR Based 18S rRNA and TAMS 1 Gene to Detect Theileria Annulata(TANUVAS, 2016-02) Saravanakumar, V.; Edith, R.; Aravindan, K.; Harikrishnan, T.J.; Azhahianambi, P.; Latha, Bhaskaran RaviOtherItem Open Access Deworming Cattle and Buffaloes(TANUVAS, 2012) Soundararajan, C.; Raman, M.; Mathialagan, P.; Sundar, S.T. Bino; Jeyathilakan, N.; Gomathinayagam, S.; Manikkavasagan, I.; Aravindan, K.; Deepika, R.B.; Subadhra, Subhra; Basith, S. AbdulOtherItem Open Access Deworming of Poultry(TANUVAS, 2012) Soundararajan, C; Raman, M.; Mathialagan, P.; Latha, B.R.; Sundar, S.T. Bino; Jeyathilakan, N.; Gomathinayagam, S.; Thenmozhi, V.; Aravindan, K.; Karthik, M.; Basith, S. AbdulArticleItem Open Access Genotyping of benzimidazole resistant and susceptible isolates of Haemonchus contortus from sheep by allele specific PCR(2016-06) Karthik, M.; Subhra, S.; Aravindan, K.; Manikkavasagan, I.Extensive and indiscriminate use of the benzimidazole class of drugs has led to the onset of anthelmintic resistance. In tropical countries like India, Haemonchus contortus is the most pathogenic parasite infecting sheep and goats. The widespread presence of resistant helminths (especially H. contortus) threatens the livestock farming. The use of various drugs has led to single nucleotide polymorphism that causes specific amino acid substitutions in b-tubulin protein of H. contortus to confer resistance. This emphasizes the need for a survey on the present status of resistance in India. In this study, allele specific PCR was employed to screen the presence of a SNP, a thymine-toadenine transversion which leads to substitution of amino acid in codon 200 of b-tubulin gene that is correlated specifically with BZ resistance. Third stage larvae (L3) from pooled faecal cultures of four organized sheep farms served as a source of genomic DNA for identification of H. contortus and further genotype analysis. A total of 1000 larvae was screened, out of which 673 larvae were identified as H. contortus. Among 673 H. contortus larvae, 539 larvae (80 %) were genotyped as homozygous resistant (rr) and remaining 134 (20 %) were heterozygous susceptible (Sr) by allele specific PCR. The concluded resistance status reasons out the failure of anthelmintic drug in treating ruminants. Immediate steps are needed to avoid further aggravation of the problem. Target selective treatment by reviewing the resistance status of individual drugs, appropriate use of anthelmintic drugs and other control strategies will provide a pragmatic option for delaying the further spread of anthelmintic resistance.OtherItem Open Access Molecular Cloning and Phylogenetic Analysis of TAMS1 Gene of Theileria Annulata Chennai Isolate(TANUVAS, 2016-02) Aravindan, K.; Saravanakumar, V.; Harikrishnan, T.J.; Edith, R.; Azhahianambi, P.; Latha, Bhaskaran RaviOtherItem Open Access PCR Based Detection of Blood Parasites in the Ticks of Cattle From Different Agro Climatic Zones of Tamil Nadu(TANUVAS, 2016-02) Edith, R.; Latha, B.R.; Saravanakumar, V.; Harikrishnan, T.J.; Azhahianambi, P.; Aravindan, K.OtherItem Open Access Status of Anthelmintic Resistance Among Small Ruminants in High Rainfall Zone of Tamil Nadu(TANUVAS, Chennai, 2011-12) Manikkavasagan, I.; Karthik, M.; Subhadra, S.; Aravindan, K.; Sundar, S.T. Bino; Basith, S. AbdulOtherItem Open Access ஆடுகளில் குடற்புழுக்களின் தாக்கம் அறிய பண்ணையாளர்களுக்கு பாமாச்சா (FAMACHA) வண்ண அட்டை(TANUVAS, 2012) Jeyathilakan, N.; Sundar, S.T. Bino; Soundararajan, C.; Gomathinayagam, S.; Raman, M.; Mathialagan, P.; Manikkavasagan, I.; Aravindan, K.; Karthik, M.; Basith, S. AbdulOtherItem Open Access மாடுகளில் குடற்புழு நீக்கம்(TANUVAS, 2012) Soundararajan, C.; Raman, M.; Mathialagan, P.; Sundar, S.T. Bino; Jeyathilakan, N.; Gomathinayagam, S.; Manikkavasagan, I.; Deepika, R..B.; Aravindan, K.; Basith, S. Abdul