Browsing by Author "Annal Selva Malar, P"
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ArticleItem Open Access Aerobic Plate Count of Milk and Dairy Products Marketed in Different Zones of Chennai(Pashupati Foundation India, 2019) Annal Selva Malar, P; Sekar, M; Porteen, K; Elango, A; Narayanan, Rita; TANUVASThe present study was envisaged to ascertain the aerobic plate count of milk and dairy products as it may imply presence of potential pathogenic organisms which is a major concern in consumer’s health point of view. A total of 567 samples, which comprises of 75 raw milk, 45 pasteurized milk and 447 dairy products were purchased from various retail outlets in different zones of Chennai. The results obtained were subjected to statistical analysis by using IDM®SPSS®Version 20.0 for windows®. Results of our study showed highest count from Chennai central zones. The microbial quality of raw milk and pasteurized milk samples tested from all the zones was found to be satisfactory. However, concentrated dairy products and khoa based dairy products tested were of unsatisfactory quality, and therefore, hygienic precautions should be taken by determining critical control points along with routine inspection of such dairy products during processing, storage, distribution and marketing.PresentationItem Open Access Screening and Identification of E. coli pathotypes by Multiplex PCR(2020-02) Annal Selva Malar, P; Sureshkannan, S; Porteen, K; Asokkumar, M; TANUVASThe study was undertaken to screen the E. coli isolates available at Department of Veterinary Public Health and Epidemiology by multiplex PCR to simultaneously group Enterohaemorrhagic E. coli (EHEC), Enteropathogenic E. coli (EPEC) and Enterotoxigenic E. coli (ETEC) pathotypes. A total number of 36 presumptive colonies isolated from milk and various dairy products were confirmed by cultural and biochemical tests according to Bergey’s manual of determinative bacteriology (Holt et al., 1994). The samples found to be positive by culture and biochemical tests were further subjected to DNA extraction by using bio basic molecular biology kit and genus specific PCR analysis was carried out targeting UspA gene (Chen and Griffiths, 1998). The isolates were further analysed for the virulence genes by using multiplex PCR using the primers specific for stx1, stx2, eae, bfp, stII and lt. (Vidal et al.2004). Among the 36 E.coli isolates, three were positive for EPEC (482bp), one was positive for ETEC (129bp) and EHEC (584bp) two were positive for both EPEC and EHEC.