Browsing by Author "ANKUR"
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ThesisItem Open Access Bioecology, Molecular Characterization of Fall armyworm, Spodoptera frugiperda (J. E. Smith) and Biochemical Basis of Resistance in Maize(MPUAT, Udaipur, 2023) ANKUR; M.K. MahlaThe result of present study shows that the infested fields by S. frugiperda were found 100 per cent in both years. Per cent infestationby S. frugiperda was found 8 to 19.33 and 11 to 23 with the mean 15.39 and 16.37 per cent or visual leaf damage rating (Davis scale 0-9) was 3 to 7 with the mean of 5.18 and 5.11, respectively in year 2021-22. During the survey two sp. Of egg parasitoid recorded Trichogramma sp. and Telenomus sp. from southern Rajasthan. The highest parasitism per cent of egg parasitoid recorded from Kapasan (31.67%) and lowest from Badgaon (16.11%) and Collected larvae are infested by parasitoid (Campoletis sp. andchelonussp.), and entomopathogens (Bt, NPV, Beauveria sp. and Metarhizium sp.) from southern Rajasthan. Highest parasitism per cent of larval parasitoid recorded from Kherwada (25%) and lowest from Rajsamand (03.33%). The highest relative density (4.68) and mean density (12.77%) of Metarhizium rileyi was recorded and minimum relative density (0.49) and mean density (1.33%) of Ophionea sp. Present studies were also carried out to understand the biochemical regulation systems in the form of nutritional, anti-nutritional and enzymatic activates in response to damage by S. frugiperda in the seedlings of different maize genotypes. The level of nutritional compounds such as total sugars, total soluble protein and starch content were significantly lower; and anti-nutritional compounds like phenol, total antioxidant and ferric ion anti reducing power (FRAP) were higher significantly higher in S. frugiperda resistant than in susceptible maize genotypes both under S. frugiperda damaged and healthy plant conditions. However, total chlorophyll and total carotenoids were significantly lower in test maize genotypes than in susceptible genotype, PHM-3. Further, the amounts of total sugars, total soluble protein, starch content, total carotenoids and FRAP inceased, and that of total chlorophyll decreased across all the test maize genotypes in response to damage by S. frugiperda. Further, present studies demonstrated that the activity of plant enzymes viz., ascorbate oxidase, ascorbate peroxidase, phenyl ammonia lyase, tyrosine ammonia lyase and catalase these enzymes was significantly higher in test maize genotypes in comparison to susceptible genotype, PHM-3, which also increased significantly in response to damage by S. frugiperda across test maize genotypes, indicating that the increase in activity of these enzymes due to S. frugiperda, feeding possibly result in physiological and chemical changes in the form of accumulation of plant defense compounds in test maize plants. Thus, the maize genotypes viz., 46131, 46132, 46136, 46137, 46138, 46139, 46140, 46141 and 46142 (Moderate resistant check) impart detrimental effects on S. frugiperda, their biochemical defense and enzymes regulation systems have been well understood, and can be utilized in breeding to derive S. frugiperda -resistant varieties of maize.The specimens S. frugiperda collected from different regions of India viz., Palampur (OR227233), Ludhiana (OR227234), Delhi (OR227235), Udaipur (OR227236), Samastipur (OR227237), Imphal (OR227238), Bengaluru (OR227239) and Namakkal (OR227240) in India (Delhi, Meghalaya, Ludhiana, Bengaluru, Pusa, Varanasi, Banswara and Pune) and abroad (Vietnam, Korea, USA, China, Pakistan, Kenya, West indies, Bangladesh, Indonesia and Africa) 98 to 100 per cent identical to these samplesThesisItem Open Access RESIDUE DYNAMICS AND DIETARY RISK ASSESSMENT OF SOME INSECTICIDE ON CABBAGE(UHF,NAUNI, 2022-11) ANKUR; SHARMA, AJAYABSTRACT The present investigations titled “Residue dynamics and dietary risk assessment of some insecticide on cabbage” were conducted in Department of Entomology, Dr. Y. S. Parmar University of Horticulture and Forestry, Nauni, Solan (H.P.) during 2021-22. Studies were carried out to evaluate the persistence of novaluron, flubendiamide and lambda-cyhalothrin on cabbage heads and soil; their risk assessment and effect of decontamination processes on the residues status of mentioned insecticides. In persistence study on cabbage heads, initial deposits at X dose of novaluron (@ 75 g a.i. ha-1), flubendiamide (@ 18.24 g a.i. ha-1) and lambda-cyhalothrin (@ 15 g a.i. ha-1) were found to be 1.143, 0.198 and 0.433 mg kg-1, respectively. At 1.25 X dose of novaluron (@ 93.75 g a.i. ha-1), flubendiamide (@ 22.8 g a.i. ha-1) and lambdacyhalothrin (@ 18.75 g a.i. ha-1), initial deposits were found to be 1.431, 0.238 and 0.541 mg kg-1, respectively. Residues of novaluron, flubendiamide and lambda-cyhalothrin reduced to half in 1.99, 3.63 and 1.46 days at X dose and 2.82, 4.64 and 1.89 days at 1.25 X dose, respectively. The residues of novaluron, flubendiamide and lambda-cyhalothrin were below limit of quantification on 15th, 10th and 7th day at X dose and 20th, 15th and 10th day at 1.25 X dose, respectively. The residues of tested insecticides were below limit of quantification in cabbage cropped soil at the time of harvest for both the doses. Dietary risk assessment studies of novaluron, flubendiamide and lambda-cyhalothrin on cabbage revealed that theoretical maximum daily intake (TMDI) was less than maximum permissible intake (MPI) and hazard quotient (HQ) was found to be less than 1 for different age categories in rural and urban areas, thus indicating all the tested insecticides to be safe for human consumption, provided proper waiting period was followed. Among various decontamination treatments, removal of outer leaves was found to be most effective (removal upto 52.74 per cent) followed by dipping in 5% NaHCO3 solution (upto 46.49 per cent) which was statistically similar to washing with lukewarm water + dipping in 1% NaCl solution (upto 42.29 per cent) followed by washing with lukewarm water + dipping in 2 % acetic acid solution (upto 35.05 per cent) followed by dipping in veggie clean solution (upto 27.2 per cent ) which was statistically similar to dipping in nimwash solution (upto 27.2 per cent) followed by lukewarm water washing (removal upto 21.28 per cent). However, washing with running tap water provide least relief of 14.93