PRESERVATION OF DOG SEMEN IN CLC LOADED SOYABEAN EXTENDER
| dc.contributor.advisor | Ahmed, K. | |
| dc.contributor.author | JOSHI, KANCHAN | |
| dc.date.accessioned | 2022-06-27T07:24:03Z | |
| dc.date.available | 2022-06-27T07:24:03Z | |
| dc.date.issued | 2022-01 | |
| dc.description.abstract | A total of 24 ejaculates were collected two times a week from four clinically healthy dogs, aged between two to six years by digital manipulation method. Four different breeds of dog viz., German Shepherd, Labrador Retriever, Golden Retriever and French Bulldog, reared singly by individual owners residing in different locations of Guwahati were used in the present study. The fresh ejaculates were examined for colour, volume, pH, mass activity, sperm motility, live sperm, sperm concentration, acrosome integrity, morphological abnormalities and HOST-reacted sperm by routine methods. The physical characteristics of freshly collected semen between dogs differ significantly for HOST-reacted sperm and sperm motility, ejaculate volume, sperm concentration and acrosome integrity but mass motility and pH did not differ significantly between animals. Total 24 ejaculates were extended in Tris-soya lecithin-citric acid-fructose and Tris-soya lecithin-citric acid-fructose + CLC extender and preserved at 4-5°C for 72 hours. The semen was assessed at 0, 24, 48 and 72 hours of preservation for sperm motility, plasma membrane integrity (HOST), morphological abnormalities and acrosome integrity. Six out of 24 ejaculates were used to assess DNA integrity and acrosome integrity at 0 and 72 hours of preservation using fluorescent stains acridine orange and FITC-PSA respectively. The sperm motility, HOST-reacted sperm and morphological abnormalities differed significantly (P<0.01) between extenders and between preservation periods. The interaction of extenders and preservation periods was non-significant in sperm motility and HOST-reacted sperm but was significant in morphological abnormalities. The addition of CLC showed significant improvement in motility, and HOST-reacted sperm (P<0.01) but morphological abnormalities were significantly higher (P<0.05) in CLC loaded semen extender. Acrosome integrity differed significantly (P<0.01) between preservation periods but the difference between extenders and interaction of extenders and preservation period was non-significant. DNA integrity of sperms differed significantly (P<0.01) between preservation periods but the difference between extenders and interaction of extenders and preservation periods was non-significant. The acrosome integrity (using FITC-PSA) of sperms differed significantly (P<0.05; P<0.01) between extenders and between preservation periods while the interaction between extenders and preservation periods was non-significant. | en_US |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810184777 | |
| dc.keywords | DOG SEMEN, CLC LOADED, SOYABEAN EXTENDER | en_US |
| dc.language.iso | English | en_US |
| dc.pages | 93p. | en_US |
| dc.publisher | College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati | en_US |
| dc.sub | Animal Reproduction, Gynaecology and Obstetrics | en_US |
| dc.theme | PRESERVATION OF DOG SEMEN IN CLC LOADED SOYABEAN EXTENDER | en_US |
| dc.these.type | M.V.Sc. | en_US |
| dc.title | PRESERVATION OF DOG SEMEN IN CLC LOADED SOYABEAN EXTENDER | en_US |
| dc.type | Thesis | en_US |