STUDY ON VIABILITY OF SKIN GRAFTS PRESERVED IN DIFFERENT MEDIA AT VARIOUS TEMPERATURES IN COW CALVES (Bos indicus)

Sonawane, Nana Arjun

STUDY ON VIABILITY OF SKIN GRAFTS PRESERVED IN DIFFERENT MEDIA AT VARIOUS TEMPERATURES IN COW CALVES (Bos indicus)

Date

1998

Authors

Sonawane, Nana Arjun

Publisher

AAU, Anand

Abstract

The effect of skin graft preservation on the viability was studied on 8 cow calves. Out of these, 6 animals were studied for room temperature (12 - 36°C) and refrigeration temperature (4°C) preservation of skin grafts in different media viz., coconut oil, cottonseed oil, groundnut oil, sunflower oil, teel oil and honey; and 2 animals were studied for cryopreservation of skin grafts in liquid nitrogen (-196°C) temperature with different concentrations of glycerol and dimethyl sulfoxide (DMSO) with honey viz., 15%, 25%, 50%, 75%, 90% and 100%. Evaluation of viability by clinical "take" of the preserved skin grafts was performed by autotransplantation on 14, 28 and 45 days intervals and the gross observations of grafted wounds were recorded on 24 hours and 10 day post-grafting. Histomorphological studies of the preserved skin grafts was performed after 24 hours, 7, 14, 21, 28, 45 and 60 days intervals. Grossly, after 24 hours and 1Oth post-grafting day all the animals where room and refrigeration temperature preserved grafts in different oils and honey were autotransplanted revealed excellent (4+) to best (3+) "take". Similar acceptance was observed in all the animals where liquid nitrogen temperature preserved grafts were autotransplanted. Historaorphologically, all oils preserved grafts recorded excellent (4+) viability upto 2 to 3 weeks and subsequently it reduced, to fair (1+) at 60 days with mild to moderate edema, degenerative changes in the epidermis and dermis. However, at refrigeration temperature they showed best (3+) viability up to 60 days. The honey preserved grafts at room temperature noted excellent (4+) viability after 24 hours and best (3+) up to 2 to 3 weeks and gradually reduced to fair (1+) at 60 days period with mild to moderate edema and degenerative changes in epidermis and dermis, whereas, at refrigeration temperature honey preserved grafts recorded best (3+) viability up to 28 days and good viability (2+) up to 60 days. The glycerol protected grafts in LN, temperature recorded excellent viability (4+) in 25%, 75% and 90%; and 25% in DMSO protection, whereas best viability (3+) was observed in 15%, 50% and 100% concentration of glycerol and 15%, 50%, 75% 90% and 100% concentration under DMSO protection.