Cytomorphological, cytochemical, cytoenzymic and ultrastructural studies on blood cells of Equus asinus
| dc.contributor.advisor | Singh, Ishwar | |
| dc.contributor.author | Meena Mrigesh | |
| dc.date.accessioned | 2016-07-12T16:22:07Z | |
| dc.date.available | 2016-07-12T16:22:07Z | |
| dc.date.issued | 2011-07 | |
| dc.description | Thesis-PhD | en_US |
| dc.description.abstract | The present study was conducted on blood cells of ten apparently healthy donkeys. The erythrocytes of donkey were 6.0±0.20 m in size. The neutrophils were round in shape and 11.75 ± 0.40m in size containing 2 to 5 nuclear lobes. The eosinophils were measured 13.35 ± 0.3m in size and appeared mulberry shape. The basophils were the largest cells among leukocytes and measured 13.85 ± 0.25m in diameter. The lymphocytes were of three different size i.e. small (mean diameter 5.8 ± 0.11 m), medium (mean diameter 7 ± 0.15 m) and large (mean diameter 9.4 ± 0.20 m). The monocytes were round in shape and measured 13.5 ± 0.17 m in size. The platelets were round, oval, fusiform and elliptical in shape. Only a few reticulocytes, siderocytes and basophilic erythrocytes were observed. The neutrophils were showing positive reaction for Periodic acid Schiff’s stain (PAS), acid phosphatase, alkaline phosphtase, beta glucuronidase, arylsulphatase. The eosinophils revealed positive reaction for PAS, Sudan black B, alkaline phosphtase, cytochrome oxidase, peroxidase, arylsulphtase and beta glucuronidase. Basophils were showing positive reaction for toluidine blue (1%) and PAS stains. Lymphocytes showed positive reaction for acid phosphtase. Lymphocytes and monocytes showed positive reaction for succinate dehydrogenase. Under scanning electron microscope erythrocytes were biconcave disc in appearance. The leukocytes were of three types. The platelets appeared irregular in out line with cytoplasmic processes. Ultrastructurally the granules of neutrophils varied in shape, size and electron density. Granules of eosinophils were mostly large, globular and electron dense. The basophils were roughly spherical in outline with pleomorphic electron dense granules in the cytoplasm. The lymphocytes were small, medium and large in sized. The monocytes were characterized by mitochondria, a few azurophilic granules and large number of pinocytic vesicles and vacuoles. The platelets had microtubules and different types of membrane bound granules. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/68667 | |
| dc.language.iso | en | en_US |
| dc.publisher | G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand) | en_US |
| dc.sub | Veterinary Anatomy | |
| dc.subject | cytology, morphology, enzymology, blood, donkeys, animal physiology, ultrastructure, blood cells | en_US |
| dc.these.type | Ph.D | |
| dc.title | Cytomorphological, cytochemical, cytoenzymic and ultrastructural studies on blood cells of Equus asinus | en_US |
| dc.type | Thesis | en_US |