Ecofriendly management of Sclerotinia wilt of French bean (Phaseolus vulgaris L.)

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dc.contributor.advisorSharma, Monica
dc.contributor.authorMeena Kumari
dc.date.accessioned2023-12-30T16:05:51Z
dc.date.available2023-12-30T16:05:51Z
dc.date.issued2023-12-28
dc.description.abstractThe fungal pathogen causing Sclerotinia wilt of French bean was isolated and identified as Sclerotinia sclerotiorum on the basis of morphological and cultural characters. Pathogenicity was demonstrated using French bean plants grown in sick pots. Seedlings exhibited symptoms such as water-soaked lesions/rotting, white fluffy cottony mycelial development, and diaback/wilting after 15 days of inoculation. The pathogen was re-isolated from artificially infected plants, and the pathogen's identity was verified. Among Biocontrol agents viz; Trichoderma harzianum and T.viridae excelled in both the dual culture test and the sick pot conditions against S. sclerotiorum. Trichoderma harzianum and T. viridae caused maximum mycelial inhibition of 71.80 and 64.25 percent, respectively. Evaluation of biocontrol agents in sick pots and the timing of biocontrol agent application in soil showed that biocontrol agents applied 7 days earlier were better to those applied simultaneously with or 7 days after the introduction of the pathogen. T. harzianum showed the maximum disease reduction (66.41%) followed by T. viridae with disease reduction (55.48%). Plant height and weight were likewise raised by T. harzianum treatment by 82.87 per cent and 65.66 per cent, respectively, and by T. viridae treatment by 80.63 per cent and 63.44 per cent, respectively. Among different abiotic resistance-inducers evaluated in sick pots against S. sclerotiorum, oxalic acid at 40 mM was found most effective in managing the disease and increasing plant weight. The activity level of key enzymes of plant defence namely phenol, chitinase and β- 1, 3- glucanase was maximum at six days after treatment with abiotic inducers and thereafter declined. Peroxidase and polyphenol oxidase activity peaked at four days following abiotic inducer treatment and thereafter dropped. The chlorophyll content was observed to be highest at 8 days following abiotic inducer treatment and then decreased.
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810205189
dc.keywordsSclerotinia wilt
dc.keywordsFrench bean
dc.keywordsPlant Pathology
dc.language.isoEnglish
dc.pages80 + vi
dc.publisherCollege of Horticulture and Forestry Dr YSP UHF, Neri, Hamirpur(H.P.)
dc.subPlant Pathology
dc.themeEcofriendly management of Sclerotinia wilt of French bean
dc.these.typeM.Sc
dc.titleEcofriendly management of Sclerotinia wilt of French bean (Phaseolus vulgaris L.)
dc.typeThesis
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