ASSESSMENT OF BIOCHEMICAL AND MOLECULAR MARKERS IN DIFFERENTIATING SUGARCANE GENOTYPES FOR SALT TOLERANCE
| dc.contributor.advisor | R.M.Naik | |
| dc.contributor.author | MAHAJAN SUCHETA TAPIRAM | |
| dc.date.accessioned | 2023-10-13T14:24:23Z | |
| dc.date.available | 2023-10-13T14:24:23Z | |
| dc.date.issued | 2010 | |
| dc.description.abstract | Sugarcane (Saccharum officinarum L.) crop shows high sensitivity to salinity at various growth stages. T.he present investigation entitled, Assessment of biochemical and molecular markers in differentiating sugarcane genotypes for salt tolerance" was undertaken with a view to analyse the effect of salt stress on different sugarcane genotypes. The sets of sugarcane were planted in normal soils. Then after one !TIOnth, the seedlings were transplanted to bigger earthen pots in normal, aline and sadie soils. The leaves from 75 days old plants were excised or the estimation of various biochemical parameters viz., proline, glycine etaine,soluble proteins, in vivo NR activity and the activity of PSCS. f urther,the leaf soluble proteins were extracted from sugarcane leaves grown on normal, saline and sadie soils and resolved on SDS-PAGE. The ifferences in isozyme forms of peroxidase and esterase were also bserved. The genomic DNA' isolated and purified from the sugarcane enotypes was amplified using different random primers.The leaf free proline content of all the sugarcane varieties increased_, the mean proline accumulation fFom 99.97 to 114.43 J..1 mole g-1 fr.wt. in saline soil and from 99.97 to 122.76 J..1 mole g-1 fr.wt. m sorlic soil. Comparison of the leaf prelme €ontents wblen gFOwn on normal, saline and sodic soils revealerl ~ferenees m feld increase in proline. Sugarcane genotypes CoM-0265,Co:-740 and Co-62175 showed an increase in proline content of 1.26, 1.25 and 1.22 .. £old in sa1ine soil and 1.34, 1.49and 1.28 -fold in sodic S0ilS, respectively. Amongst the promising clones, CoM -08011 appeared to 1De sa1t tolera.I'lt showing an increase in leaf proline content of 1.1 ~and 1.25 fold under salline and sodic conditions. It thus appears that fold increase in pF®line level is a sensitive parameter while evaluating salt toleFance and pFoline accumulatoin is an adaptive parameter unde:r stress c0naition. The early maturing and high sugar genetypes viz., CoC-671 and Co-940 12 appeared to be salt susceptible. ·A significant increase in mean g~cine ,betaine content was recorded with 4.34-fold increase in saline soils and 16.63 fold in sodic soils. A maximum fold iiacrease in glyci~e betaine content of 10.44 and 51.06 fold was reco~ded in CoM-0265, followed by Co-7 40 with a fold increase of 8.19 and 27.28 in saline and sodic soils~ respectively . The early maturing aE.d nigfu suga:r centaining sugarcane genotypes,.,CoC-671 and Co-94012 showed the minimum fold increase of 2.45 and 1.68, respectively when grown on saline soils and 7.42 and 6.77 when grown on sodic soils.The soluble protein content also increased under stress aE.dWlehighest fold increase ef 2.52 and 4.18 was observed in CoM-0265 in saline and sodic soils,respeetively. 1'he increase in free proline content is not due te proteolysis but rather due to increased de-novo synthesis. The in vivo leaf nitrate reductase activity was drastically reduced when grown on saline and sodie soils. A :m.inimum reduction in the in vivo NR activity from 0.62 in normal soH to 0.54 J..1 mole N02~ fromed g-1 fr.wt.hr-1 in saline soil was recorded in CoM-0265, followed by a reduction of 14.46 % in Co-740. It is interesting to note that early aturing and high sugar genotypes, CoC-671 and Co-94012 showed igher in vivo NR activity of 1.63 and 1.61 j..1 mole N02- formed g -~ r.wt.hr-1 , respectively when grown on normal soils. However , the eduction in the in vivo NR activity was significant to the extent of 32.90 Yo in CoC-671 and 43.52% in Co-94012 in saline soils and 62.64% and 4.66% in sodic soils. | |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810198832 | |
| dc.language.iso | English | |
| dc.pages | 145pp | |
| dc.publisher | MPKV,Rahuri | |
| dc.sub | Agricultural Biochemistry | |
| dc.theme | Agricultural Biochemistry | |
| dc.these.type | M.Sc | |
| dc.title | ASSESSMENT OF BIOCHEMICAL AND MOLECULAR MARKERS IN DIFFERENTIATING SUGARCANE GENOTYPES FOR SALT TOLERANCE | |
| dc.type | Thesis |