STUDIES ON GENETIC DIVERSITY OF DIAMONDBACK MOTH, Plutella xylostella, (L.) (Lepidoptera) IN NORTH INDIA
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Date
2017-10
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UHF,NAUNI
Abstract
ABSTRACT
Geographic variations among different populations of Plutella xylostella collected from eight
different locations viz. Solan, Palampur and Theog (Himachal Pradesh), Fatehgarh Sahib (Punjab),
Sharnapur (Uttar Pradesh), Pantnagar (Uttarakhand), Hisar (Haryana) and Delhi were studied.
Standard molecular technique RAPD-PCR was also utilized to study the variations among different
populations. In addition to this the variations regarding different biology and life tables were
investigated in the laboratory. The RAPD-PCR studies were conducted with 20 random primers out of
which 12 produced amplified PCR products. A total of 73.03 per cent polymorphism was observed
among eight populations of P. xylostella. 100 per cent polymorphism was observed with OPA-16 and
OPAB-10 primers whereas, minimum polymorphism (40.00%) was observed in OPD-14 which
produced 5 amplified bands. The values of similarity coefficient ranged from 0.50 to 0.70. Maximum
similarity was observed between Solan and Theog populations (70.00), whereas, minimum similarity
was observed between Delhi and Pantnagar populations of P. xylostella. The total developmental
period was minimum in the Fatehgarh Sahib population (13.74 days) followed by the Theog,
Pantnagar, Saharanpur, Solan, Delhi and Hisar, Palampur populations (14.72, 15.00, 15.73, 15.85,
16.92, 17.20 and 17.65 days, respectively. The life table studies revealed that the intrinsic rate of
natural increase (rm) was the highest in the Theog population (0.242) followed by the Palampur
(0.236), Saharanpur (0.232), Fatehgarh Sahib (0.221), Pantnagar (0.218), Solan (0.211) and Delhi
(0.195) population, whereas it was least in the Hisar population (0.189). Continuous exposure of
indoxacarb and chlropyrifos may result in biologically superior strain of diamonback moth as revealed
by the increase in LC50 value from 17.06 ppm to 43.07 ppm for Solan and 21.70 ppm to 60.53 ppm to
Fatehgarh Sahib population with 2.56 and 2.80 folds as compared with parent generation for
indoxacarb. LC50 value increase from 102.67 ppm to178.61 ppm for Solan and 155.81 to 248.15 ppm
with 1.74 and 1.59 folds as compared with parent generation for chlorpyrifos.
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