DEVELOPMENT OF MUTANTS IN BANANA CULTIVAR NEY POOVAN RESISTANT TO WILT DISEASE CAUSED BY FUSARIUM OXYSPORUM F. SP. CUBENSE THROUGH IN-VITRO MUTAGENESIS OF EMBRYOGENIC CELLS
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Date
2017-09
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University of Horticultural Sciences, Bagalkot. (College of Horticulture, Bengaluru).
Abstract
Investigations on “Development of mutants in banana cultivar, Ney Poovan resistant to
wilt disease caused by Fusarium oxysporum f. sp. cubense through in-vitro mutagenesis of
embryogenic cells” were carried out during 2015-17 at Division of Biotechnology and Division of
Plant Pathology, Indian Institute of Horticultural Research (ICAR-IIHR), Bengaluru. Embryogenic
cell suspension of banana was mutagenated through chemical and physical methods by Ethyl
Methane sulphonate and gamma irradiation, respectively. Effect of treatment duration of EMS
(0.2%, v/v) on plant regeneration capacity of ECS was remarkably high with 10 min EMS
treatment followed by control and decreased with increase in treatment duration. Gamma
irradiation doses on plantlets regeneration capacity revealed that the no. of viable embryos was
highest in control (1044) and it decreased with the increase in the strength of gamma irradiation.
LD50 for EMS duration was 20 to 30 min and gamma irradiation was 30 Gy. Embryos obtained
were screened through toxin fusaric acid followed by Foc culture filtrate and fusaric acid
supplemented media. Twelve out of 200 in Ney Poovan, EMS-10 min and 17 out of 200 in Ney
Poovan EMS-15 min treatment survived the toxin media. Survived plants from in vitro screening
subjected to pot screening under polyhouse condition yielded two each mutagenated banana, from
10 min EMS group and 15 min EMS group. There was significant elevation in PAL and
Peroxidase activity in some of the mutants in the defence related enzyme assay. Four mutagenated
plants found to be tolerant to Fusarium wilt from the present investigations.