Expression analysis of flowering switch factors using candidate gene approach in mango
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Date
2020
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Punjab Agricultural University, Ludhiana
Abstract
Mango (Mangifera indica L.) is a major fruit crop of the tropical and subtropical regions of the world. Some of the varieties especially grown in north western India are prone to alternate/biennial/irregular bearing which is controlled genetically. For the investigating this problem, the gene responsible for floral induction i.e. Flowering Locus T (FT) gene was isolated from the leaf tissues of the two mango varieties, Amrapalli and Dashehari. After confirmation and characterization, gene sequence (approximately 1.5 kb) was isolated. The gene sequence obtained for Amrapalli was 78 base pairs longer than the gene sequence of Dashehari. Blastn analysis of the isolated FT gene showed high similarity nucleotide sequence identity from different mango cultivars as well as other perennial fruit trees. The transcript expression of FT was evaluated in leaf tissues during early stage (mid-January) i.e. before flowering, mid stage; prior to induction of flowering (mid-February) and late stage; prior to flower bud initiation (early-March) using semi quantitative method in two consecutive years i.e. 2017 and 2018. Its expression level was more in mid stage as compared to other two stages. The expression level was more in Amrapalli in the year 2018 due to its regular bearing habit.