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Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

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2010 - 201425
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Subject: Vegetable Science × End date: 2014 × Start date: 2010 ×
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    ThesisItemOpen Access
    Studies on in vitro plant regeneration in Capsicum (Capsicum annuum L.)
    (CCSHAU, 2014) Hegde, Vivek; Partap, P.S.
    Development of an efficient plant regeneration system is a pre-requisite for any plant biotechnological approach for crop improvement. In this regard the in vitro regeneration from cotyledon and hypocotyl explants from aseptically raised seedlings of popular Capsicum F1 hybrids Bharat and Indra was achieved. The anther culture was also tried to produce double haploids. Seeds of both hybrids were exposed to different treatments for proper germination. The treated seeds were decontaminated and placed in a half-strength MS medium and then incubated in 16h photoperiod for germination. The seed soaked in distilled water along with GA3 at 2 mg/l for two days prior to sowing had more pronounced effect on both capsicum hybrids recording maximum germination (90.45 and 84.59 %, respectively) and that also in minimum number of days (9.67 days and 10.33 days, respectively). Tissue culture responses to morphogenesis varied with the genotypes, source of explants, type of explants and combinations of growth regulators used. Per cent regeneration (96.30%), number of shoots per explants (4.56) and per cent elongation (82.10%) was maximum in hybrid Indra from cotyledons having abaxial side in contact with growing medium supplemented with zeatin at 7.5 mg/l along with GA3 at 2.0 mg/l. Cent per cent rooting, optimum number of roots (27.56 and 23.65, respectively) and root length (4.94 cm and 7.71 cm, respectively) were observed when regenerated shoots cultured on MS media supplemented with IBA at 0.5 mg/l in both the hybrids. The survival percentage of regenerated plantlets was higher in Indra (92.31%) as compared to Bharat (85.71%) during hardening in pots containing sterile mixture of coco-peat and vermiculite (1:1) and thereafter in pots containing soil. Anthers of both hybrids were collected at three different stages of microspore development and cultured on medium supplemented with different ratios of growth regulators and additives. The effect of heat treatment at 35oC for seven days on androgenesis was also examined. Androgenic callus induction was best on heat treated anthers cultured on media supplemented with zeatin at 1.0 mg/l along with 2,4-D at 0.2 mg/l and AgNO3 at15 mg/l in both the hybrids (54.02% and 60.92%, respectively) and hybrid Indra responded better than Bharat. The effect of different cytokinins (BAP, kinetin, TDZ and zeatin) along with NAA on regeneration of androgenic callus was also studied, however, shoot induction could not be achieved. Hence, greening of callus in particular medium was recorded. The MS media supplemented with kinetin at 7.5 mg/l along with NAA at 0.2 mg/l recorded maximum per cent greening of callus i.e. 53.33% and 51.11% in hybrid Indra and Bharat, respectively.
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    ThesisItemOpen Access
    Molecular Diagnosis Of Antimicrobial Resistance Associated With Bovine Staphylococcal Mastitis
    (Chaudhary Charan Singh Haryana Agricultural University; Hisar, 2010) Sindhu, Neelesh; Sridhar
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    ThesisItemOpen Access
    Effect of manures and biofertilizers on growth and yield of garlic
    (CCSHAU, 2012) Ramesh Kumar; Avtar Singh
    The experiment entitled “Effect of manures and biofertilizers on growth and yield of garlic” was conducted at Research Farm of the Department of Vegetable Science, CCS HAU, Agricultural University, Hisar during winter season of 2009-10. The experiment comprised the 15 treatments (manures and biofertilizers) and was laid out in a Randomized Block Design with three replications. Net plot size was kept 2.25× 2 m. Ten competitive plants were observed randomly from each experimental treatment to record data on various parameters that were influenced by different manures and biofertilizers. Plant height, number of leaves per plant, average length of leaves, average breadth of leaves were found maximum with treatment T6 followed by T11 at 45, 60 and 90 DAS and same trend was observed in case of average bulb diameter, bulb neck thickness, average bulb weight, number of clove per bulb, clove size, total plant biomass and shoot weight per plant. The yield parameters of garlic were significantly influenced by different manures and biofertilizers. The yield of garlic was found maximum with treatment of T11 (124.24 q/ha) which was on par with T9 (124.05 q/ha). The lowest garlic yield was observed with T15 (114.06 q/ha). HG-17 produced highest leaf dry matter (%), Bulb dry matter (%) and total soluble solids along with treatment of T6 followed by T11 and T14.
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    ThesisItemOpen Access
    Studies on intercropping and crop geometry in organic production of broccoli (Brassica oleracea var. italica)
    (CCSHAU, 2014) Yadav, Lalu Prasad; Singh, Avtar
    The research investigation entitled “Studies on intercropping and crop geometry in organic production of broccoli (Brassica oleracea var. italica)” was conducted at the research farm and laboratory of Department of Vegetable Science, CCS Haryana Agricultural University, Hisar during 2012-13 and 2013-14. The experiment was laid out in split plot design replicated thrice with three different organic manure and two spacing as main plot treatments and five intercrops including sole crop of broccoli as sub-plot treatments, thus making a total of thirty treatment combinations, from the various parameters recorded number of leaves, days to first and 50 per cent harvesting, maximum weight of main head (g), girth of head (cm), number of sprouts per plant, weight of sprouts per plant (g), yield of sprouts per plant (g), total yield per plant (g), total yield (q/ha), plant biomass (g), mineral content in whole plant, vitamin A (mg/100g) and vitamin C (mg/100g) of broccoli were maximum in the M2S1C1 While, phosphorus content (mg/kg) in plant in the treatment M3S1C1. However, plant height (cm), minimum days to harvesting and yield (q/ha) of intercrops was maximum in the M2S2C4 treatment. So, it is concluded from the present investigation that application of vermicompost under a spacing of 45 x 45 cm the sole crop of broccoli (M2S1C1) recorded higher total yield (303.9 q/ha). The highest total yield of greens and roots was recorded with the application of vermicompost under paired row spacing and radish as the intercrop planted in broccoli (M2S2C5) followed by the application of poultry manure also under paired row planting system and radish as intercrop (M3S2C5). The maximum net return (Rs. 162795) was obtained under the M2S1C4 treatment, however, benefit: cost ratio (1: 0.84) was under the treatment M3S1C4, which was statistically at par with the M2S1C4 treatment (1: 0.83).
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    ThesisItemOpen Access
    Effect of manures, bio-fertilizers and mulching on growth and yield of potato
    (CCSHAU, 2013) Rajesh kumar; Avtar, Singh
    The experiment entitled “Effect of manures, bio-fertilizers and mulching on growth and yield of potato” was conducted at Research Farm of the Department of Vegetable Science, CCS Haryana Agricultural University, Hisar during winter season of 2010-11 and 2011-12 to find out the sutaible level of manures and mulching for getting higher yield as well as keeping quality of potato tubers at ambient room temperature. The potato variety used for the investigation was Kufri Bahar. The treatments comprising two levels of mulching and non- mulching and sixteen levels of organic manures were laid out in a split plot design with three replications keeping a net plot size of 3.0 x 3.0 m. The ten competitive plants were selected randomly from each treatment to record data on various parameters, which were influenced significantly by different levels of mulching and organic manures. The maximum value for plant height at 45, 60, 75 and 90 days after planting, number of stems per hill, number of leaves per stem, leaf weight per hill (g), stem weight per hill (g), leaf area index, number and weight (kg) of <25, 26-50, 51-75 and >75 g size tubers, total number and weight of tubers (kg / m2) total and marketable tuber yield (kg/m2 and q/ha) were recorded maximum with the application of vermi-compost @ 6.0 t/ha + biofertilizers + ( Azotobactor + PSB). However, the number and weight (kg) of up to 25 g size tubers was noticed maximum with out organic manures application. Dry matter and starch content of potato tubers (%) at harvest was recorded maximum with vermi-compost @ 6.0 t /ha + bio-fertilizers + ( Azotobactor + PSB), while the total ethanol soluble sugars content of potato tubers at harvest on dry weight basis (mg/100 g) was minimum vermicompost @ 6.0 t /ha + bio-fertilizers + ( Azotobactor + PSB). The minimum value for physiological loss in weight and decay loss of potato tubers (%) at 15, 30, 45 and 60 days after harvest was recorded with vermi-compost @ 6.0 t /ha + bio-fertilizers + ( Azotobactor + PSB). The interaction effect of mulching and manures showed significant variation for leaves weight, leaf area index, tuber yield and keeping quality parameters. Significantly the highest values for leaves weight per hill (g), leaf area index, weight (kg) of <25, 26-50, 51-75 and >75 g size tubers per square meter, total weight of tubers (kg) per square meter were registered with the treatment combination of mulching and vermi-compost @ 6.0 t /ha + bio-fertilizers + (Azotobactor + PSB) . Similarly, minimum value for physiological loss in weight and decay loss of potato tubers (%) at 15, 30, 45 and days after harvest was noted with treatment combination of mulching and application vermi-compost @ 6.0 t/ha + bio-fertilizers + (Azotobactor + PSB). The two years results suggest that the mulching in combination with vermi-compost @ 6.0 t /ha + bio-fertilizers + (Azotobactor + PSB) has shown the best treatment combinations for potato production and storage at ambient room temperature, respectively under semiarid conditions of Hisar (Haryna).
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    ThesisItemOpen Access
    Effect of irrigation and potash levels on yield and keeping quality of potato (Solanum tuberosum L.)
    (CCSHAU, 2012) Adhikari, Ram Chandra; Rana, M.K.
    The experiment entitled “Effect of irrigation and potash levels on yield and keeping quality of potato (Solanum tuberosum L.)”was conducted at Research Farm of the Department of Vegetable Science, CCS Haryana Agricultural University, Hisar during winter season of 2010-11 and 2011-12 to find out the optimum level of irrigation and potash for getting higher yield as well as keeping quality of potato tubers at ambient room temperature. The potato variety used for the investigation was Kufri Bahar. The treatments comprising four levels of irrigation (25, 30, 35 and 40 mm CPE) and four levels of potash (0, 100, 125 and 150 kg/ha) were laid out in a split plot design with three replications keeping a net plot size of 3.6x3.6 m. The ten competitive plants were selected randomly from each treatment to record data on various parameters, which were influenced significantly by different levels of irrigation and potash. The leaves weight per hill (g), leaf area index, weight (kg) of >25-50, >50-75 and > 75 g size tubers,total tuber yield (kg/m 2 and q/ha) and marketable tuber yield (kg/m 2 and q/ha) were registered significantly maximum with irrigation level 35 mm CPE. Likewise, the values for physiological loss in weight and decay loss of potato tubers (%) at 15, 30, 45 and 60 days after harvest was lowest with irrigation level 40 mm CPE. The maximum value for plant height at 45, 60, 75 and 90 days after planting, number of stems per hill, number of leaves per stem, leaves weight per hill (g), stem weight per hill (g), leaf area index, number and weight (kg) of >25-50, 50-75 and >75 g size tubers, total number and weight of tubers (kg) per meter square and total and marketable tuber yield (kg/m 2 and q/ha) were recorded maximum with the application of potash @ 150 kg/ha. However, the number and weight (kg) of up to 25 g size tubers was noticed maximum with no potash application. Dry matter and starch content of potato tubers (%) at harvest was recorded maximum with potash application @ 150 kg/ha, while the total ethanol soluble sugars content of potato tubers at harvest on dry weight basis (mg/100 g) was minimum with potash 150 kg/ha. The minimum value for physiological loss in weight and decay loss of potato tubers (%) at 15, 30, 45 and 60 days after harvest was recorded with potash application @ 150 kg/ha. The interaction effect of irrigation and potash levels showed remarkable variation for leaves weight, leaf area index, tuber yield and keeping quality parameters. Significantly the highest values for leaves weight per hill (g), leaf area index, weight (kg) of >25-50, 50-75 and >75 g size tubers per square meter, total weight of tubers (kg) per square meter were registered with the treatment combination of irrigation level 35 mm CPE andpotash 150 kg/ha. Similarly, significantly the minimum value for physiological loss in weight and decay loss of potato tubers (%) at 15, 30, 45 and days after harvest wasnoted with treatment combination of irrigation level 40 mm CPE and potash application @ 150 kg/ha. The two years results suggest that the irrigation level 35 mm CPE in combination with potash @ 150 kg/ha and irrigation level 40 mm CPE with potash 150 kg/ha have shown the best treatment combinations for potato production and storage at ambient room temperature, respectively under semiarid conditions of Hisar (Haryna).
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    ThesisItemOpen Access
    Assessment of heterosis and combining ability of advance lines in okra [Abelmoschus esculentus (L.) Moench]
    (CCSHAU, 2010) Juvekar netaji shivaji; Yadav, A.C.
    The present field investigation entitled “Assessment of heterosis and combining ability for advance lines in okra [Abelmoshcus esculentus (L.) Moench] was undertaken at Vegetable Research Farm, Department of Vegetable Science, CCS Haryana Agricultural University, Hisar during rainy season 2009-2010. Fifteen females, three males and their forty five hybrids along with standard check Varsha Uphar were evaluated in a randomized block design with three replications. Data were recorded for fruit yield and its associative traits. The analysis of variance revealed that significant differences were observed for all the characters studied, thereby indicating considerable variability among the genotypes. The extent of heterosis varied from cross- to- cross for all the characters studied. Marked heterosis was observed for fruit yield and its associative traits. However, two cross combinations namely HRB-146-2-1 x Hisar Unnat and HRB-146-1-1-1 x Hisar Navin manifested maximum desirable heterosis for days to 50 per cent flowering, days to first picking, number of fruits per plant and fruit yield, that may be utilized to exploit hybrid vigour for commercial hybridization programme. Combining ability study indicated that the both additive and non additive gene actions governed the inheritance of all the characters studied. General combining ability effects advocated that female parents viz. HRB-141-1, HB-03-25-2, Raj-12 and HRB-146-2-1 were observed good general combiners for fruit yield and its associative traits. None of the male parents identified as good general combiner. Per se performance and gca effects of parents exhibited very close relationship. The estimates of specific combining ability effects suggested that two crosses, HRB-150-1-1 x Hisar Unnat and HB-06-1 x JNDOL-03 showed high sca effects for fruit yield and its component traits. Hence, recurrent selection by intermating of most desirable segregants can be adopted for the improvement programme in order to utilize additive and non additive genetic variances controlling inheritance of different characters studied. Genotypic correlation study revealed that the fruit yield showed highly significant and positive association with number of fruits per plant and fruit weight. Besides, fruit diameter, fruit length and plant height had positive correlation with fruit yield. Path analysis indicated that positive direct effect on fruit yield through number of fruits per plant followed by fruit weight and fruit length. Therefore, simple selection method will be effective for crop improvement programme as the expression of these characters is controlled by additive gene action.
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    ThesisItemOpen Access
    Seed germination studies in bitter gourd (Momordica charantia L.)
    (CCSHAU, 2010) Narender Kumar; Partap, P.S.
    Seed germination study of bitter gourd varieties Pusa Do Mausmi and Pusa Vishesh under laboratory and field conditions using eight chemicals and three seed soaking durations was carried out in March and August, 2009. Analysis of variance revealed highly significant differences among the treatment effects for different characters in both the varieties for respective seed soaking durations. Test weight of sampled seeds was 17.99g (Pusa Do Mausmi) and 16.96g (Pusa Vishesh). In both sets of experiments varieties differed for all the characters in respective seed soaking durations under laboratory and field conditions where some interactions were also significant. The laboratory seed germination and seedling field emergence for both the sets of experiments and all the three seed soaking durations were found similar but field emergence was lesser than laboratory seed germination in all the treatments investigated. In both the sets of experiment GA3 50ppm followed by KCl (1%) and ZnSO4 (1%) gave significantly higher seed germination then the canal water control treatment in both the varieties. Pusa Vishesh had better germination than Pusa Do Mausmi, however, field emergence was at par in both the varieties. Field emergence (%) and field emergence index were recorded maximum with GA3 50ppm/boron 25ppm and or GA3 50ppm/KCl (1%) and KNO3 (1%) and ZnSO4 (1%). It was lowest with canal water and IBA 75ppm. The other associated parameters of seed quality i.e. seedling length (root/shoot), seedling fresh/dry weight, seed vigour index-I and II were also influenced by the chemical treatments and followed almost the same pattern as of seed germination and seedling field emergence.Seed soaking duration of 12h was better than 24 and 36 h seed soaking.The seed germination, field emergences/index, seedling size, and seed vigour index were reduced with aging of the seeds. The patterns of field emergence, which showed non-to-multi peaks was not consistent for the chemical response, seed soaking duration and also for the crop season. GA3 50ppm, KCl (1%) and KNO3 (1%) with 12h seed soaking appeared most promising for enhancing seed germination/field emergence of bitter gourd varieties.
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    ThesisItemOpen Access
    Effect of biofertilizers and organic manures on seed yield and quality of fenugreek
    (CCSHAU, 2010) Chaudhary, Rajesh; Tehlan, S.K.
    The field experiment entitled “Effects of biofertilizers and organic manures on seed yield and quality of fenugreek” was conducted at Research Farms, Department of Vegetable Science and laboratories of Department of Seed Science and Technology, CCS Haryana Agricultural University, Hisar during rabi season of 2008-09. The results revealed that application of biofertilizers, organic manures and their combinations significantly influenced the growth, yield and quality parameters of fenugreek except days taken to 50 percent flowering. Seed inoculation with Rhizobium alone or in combination with PSB statistically produced non significant increase in yield over control. However, seed inoculation with Rhizobium along with application of different organic manures was found superior over PSB in term of growth, yield and quality parameters of fenugreek. Similarly, application of organic manures along with seed treatment with biofertilizers (Rhizobium or Rhizobium + PSB) perform better than sole application of organic manures. The treatment combinations viz. Rhizobium + PSB + farmyard manure, Rhizobium + farmyard manure and Rhizobium + PSB + vermicompost produced higher seed yield with better quality which were at par with recommended dose of NPK.