Dr A M BaruahChoudhury, B. H.2017-03-082017-03-082015http://krishikosh.egranth.ac.in/handle/1/5810004468Twenty five indigenous leafy vegetables were selected on the basis of their popularity of uses and collected from various places of Jorhat district. These plants were used for biochemical and molecular investigation. These plant species showed significant variations in various biochemical constituents such as moisture (76.40- 92.77%), ash (7.80-17.70%), K (0.64- 9.58%), Na (52.0-233.00 mg/100 g), Ca (200.7-2104.96 mg/100 g), P (38.00-603.00 mg/100 g), Fe (31.00-401.00 mg/100 g, Cu (0.003 -0.020%), Zn (0.013-7.55 mg/100 g), Mn (12.00-132.00 mg/100 g), As (0.00-1.70 mg/100 g), crude fat (1.13-7.10%), crude fibre (2.50-26.10%), crude protein (15.77-39.38%), total free amino acid (129.27-992.00 mg/100 g), starch (18.78-56.42%), TSS (7.37-18.37%), reducing sugar (4.19-18.58%), non-reducing sugar (0.72-7.36%), soluble protein (1.10-4.13%), vitamin C (39.00-119.67 mg/100 g), riboflavin (0.17-8.80 mg/100 g), thiamine (0.00-0.08 mg/100 g), niacin (0.15-2.63 mg/100 g), total alkaloid (0.13-3.23 mg/100 g), saponin (0.23-0.61%), tannin (0.37-2.82%), phytic acid (0.03-0.32%), oxalate (0.02-0.13%), total phenol (0.25-2.2%), free radical scavenging activity (74.81-91.72%), total flavanoid (0.86-3.40%), total flavanol (0.33-1.63%), super oxide dismutase specific activity (0.11-0.24 unit min-1 mg-1protein), catalase specific activity (0.053-0.13 unit min-1 mg-1protein), peroxidase specific activity (0.01-0.08 unit min-1 mg-1protein). The RAPD analysis of Alternanthera spp. land races revealed the genetic diversity among the land races. The maximum level of polymorphism was generated by OPA-04 primer (100%) followed by OPB-05 primer (87.50%). The RAPD analysis of Amaranthus spp. land races revealed the genetic diversity among the land races. The maximum level of polymorphism was generated by OPB-01 primer (71.42%) followed by OPB-07 primer (66.67%). The RAPD analysis of Chenopodium spp. land races revealed the genetic diversity among the land races. The maximum level of polymorphism was generated by OPA-04 primer (100%) followed by OPB-02 primer (77.21%). The RAPD analysis of Enhydra spp. land races revealed the genetic diversity among the land races. The maximum level of polymorphism was generated by OPC-01 primer (100%) followed by OPB-01 primer (80.00%). From the present study it was observed that all the indigenous leafy vegetables are rich in various nutritionally significant biochemical constituents and genetic diversity exists among the land races of studied plant species though no distinct morphological variation was seen.ennullThesis