Ravendra KumarRandeep Kumar2019-06-192019-06-192019-05http://krishikosh.egranth.ac.in/handle/1/5810109052In present study the preliminary qualitative and quantitative phytochemical analysis along with pharmacological activities like in-vitro antioxidant and anti-inflammatory activity and pesticidal activity like insect antifeeding and herbicidal activity of essential oil and different plant extracts of Limnophila indica (L.) Druce were evaluated. The yield of different samples obtained were 0.98%, 8.16%, 1.1% and 3.3% for essential oil, methanol, chloroform and hexane extracts respectively. The presence and absence of various secondary metabolites like alkaloids, saponins, phenols, flavonoids, and diterpenes were observed in all the samples. The GC-MS analysis reveals the identification of 90.9%, 74.3%, 72.9% and 84.3% compounds of the total samples composition of essential oil, methanol, chloroform and hexane extracts respectively. Major compounds like epi-cyclococlorenone (27.7%), _-monolinolein (12.9%), 4,5-dimethyl-1,2,3,6,7,8,8a,8b-octahydrobiphenylene (18.3%) and aristolone (40.3%) were identified in essential oil, methanol, chloroform and hexane extracts respectively. The quantitative evaluation of total phenolics content (TPC), total flavonoid content (TFC) and ortho dihydric phenol (ODP) content of all the extracts were calibrated in terms of gallic acid, catechin and catechol equivalents respectively and resulted the composition in order of chloroform extract > methanol extract > hexane extract. The in-vitro antioxidant activity was performed in terms of DPPH radical scavenging, NO radical scavenging, metal chelating and reducing power effect against standard antioxidants like BHT, ascorbic acid, citric acid and catechin and the results analysed to be very effective with a broad range of inhibition with potent IC50 values. Also, the in-vitro anti-inflammatory activity were tested as a measure of inhibition of protein denaturation method against standard diclofenac sodium and evaluated to be the significantly different range of inhibition as tested at different doses levels. Evaluation of insect antifeeding activity were done with non-preferential leaf dip method against Spilosoma obliqua (Bihar hairy caterpillar) resulted into a significantly diverse range of antifeedant index. Assessment of herbicidal activity in terms of inhibition of percent germination, inhibition of coleoptile length and inhibition of radicle length as tested on the seeds of Raphanus sativus revealed the significant properties in the essential oil and the plant extracts of the tested plant as analyzed against the standard herbicide pendimethalin. All the inhibition data were tested to be significantly different as analyzed via two factor analysis with replication (p<0.01 and p<0.05).ennullThesis