H. A. PrameelaHANUMANTH KORAVAR2023-01-142023-01-142022-12-29https://krishikosh.egranth.ac.in/handle/1/5810192310ThesisFrench bean is оne of the majоr important vegetable pulse, leguminous crops grown throughout the world. Bean common mosaic virus (BCMV) infected french bean samples strongly reacted with BCMV specific antisera in DAS ELISA. An approximately 750 bp PCR product corresponding to coat protein (CP) region of virus was obtained through RT-PCR. Virus was 90- 100 per cent mechanically transmissible to french bean cultivar Moraleda with characteristic symptoms like vein clearing, uneven leaf lamina, twisting of newly emerged trifoliate leaves, puckering, blistering, mosaic patches on leaves and stunted growth of the plants. Myzus persicae found more efficient (73.33 %) in transmission of virus as compared to Aphis craccivora (53.33 %) while seed transmission ranged from 22.22 to 34.00 per cent. The aphid, M. persicae and A. craccivora transmitted BCMV with transmission range of 70.00 and 50.00 per cent when given pre-acquisition starvation period, AFP, IFP and RP period of 60 min, 20 min, 20 min and 20 min respectively. However, maximum per cent transmission of 70 per cent (M. persicae) and 50 percent (A. craccivora) was recorded when 15 aphids were used. The BCMV produced systemic symptoms in different leguminous plant species like, french bean, pea, green gram, horse gram, field bean, black gram, soybean, cowpea, pole bean, moth bean and brinjal whereas Chenapodium amaranticolor and Nicotiana rustica showed necrotic local lesions. The plants belonging to Cucurbitaceae were not infected with BCMV. The presence of BCMV from different host plants was confirmed by DAS-ELISA using BCMV specific antisera.EnglishThesis