Dhillon, SantoshVashisth, Medhavi2016-10-052016-10-052014http://krishikosh.egranth.ac.in/handle/1/80027Stripe rust (yellow rust), caused by Puccinia striiformis Westend. f. sp. tritici Eriks., is one of the most devastating diseases of wheat throughout the world. Marker assisted breeding is the most economical and environmentally acceptable method to control stripe rust. The present investigation was carried out to evaluate F2 population derived from WH711 and WH542 varieties of wheat for reaction to yellow rust, traits contributing to yield and SSR analysis. WH711 (susceptible to yellow rust) × WH542 (resistant to yellow rust) F2 population when screened against yellow rust reaction, fitted in asegregating ratio of 9:3:3:1 for highly resistant, moderately resistant, moderately susceptible and highly susceptible plants, suggesting the digenic effect (two gene segregation) controlling the respective trait. Correlation analysis revealed that biological yield per plant (0.891) had the highest direct contribution towards grain yield per plant followed by number of tillers per plant (0.816). Out of 100 SSR markers used, 81 SSRs showed amplification, out of which 17 SSRs (including four Yr specific SSRs specific for Yr genes viz. Yr1, Yr16, Yr36, YrH52) produced polymorphic bands while 64 SSRs produced monomorphic bands. Size of amplified products in two different genotypes ranged from 86 bp to 288 bp. WH711 × WH542 plants showed heterozygosity of 38% and homozygosity of 62%. Though no marker was found linked to the rust resistance but NTSYS-PC UPGMA cluster tree analysis led to the grouping of 120 F2 plants in two major groups at a similarity coefficient of 0.55, major group I containing 22 F2 plants and WH711while major group II containing 97 F2 plants and WH542, thus indicating F2 population is more inclined towards WH542.enPlanting, Diseases, Wheats, Genes, Grain, Genetics, Yields, dna, Electrophoresis, AllelesThesis