Dr. M. K. JhalaCHANNABASAYYA HIREMATH2017-07-142017-07-142008http://krishikosh.egranth.ac.in/handle/1/5810025199Chicken infectious anemia virus (CIAV) is an economically important and contagious avian pathogen with a worldwide distribution, causing chicken infectious anemia (CIA) in chickens. CIAV is a non-enveloped, icosahedral virus having a single stranded circular DNA genome and belongs to genus Gyrovirus, within the family Circoviridae. In the present study, the suspected CIA incidences were confirmed by PCR applied to different affected tissues collected from clinically affected birds from poultry flocks in and around Anand district of Gujarat. To study the extent of genetic variability of the field CIAV, the nucleotide sequences of VP1, VP2 and VP3 genes of CIAV were determined directly from the cloned PCR products, without isolating the virus in cell culture to obviate the potential genetic nucleotide changes that can possibly occur during cell culture passage. The gene sequences and the deduced aminoacid sequences of VP1, VP2 and VP3 of CIAV were compared with the other Indian and foreign sequences available in GeneBank and phylogenetic analysis based on nucleotide and deduced aminoacid sequences of VP1 was carried out to access the genetic distance of CIAV circulating in the field.enVeterinary MicrobiologyAnalysisThesis