Kolhe A. V.ARYA, KRISHNA2018-10-072018-10-072018-06-14ARYA KRISHNA. (2018). In vitro assessment of some IPM components against major sucking pests of cotton. Department of Agricultural Entomology, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola. M. Sc. 2018. Print. xii, 69p. (Unpublished).http://krishikosh.egranth.ac.in/handle/1/5810074923The present study deals with the predatory response of ladybird beetles and estimate the parameters of the type of response showed by it. Toxicological studies of imidacloprid and acephate was done to know the median lethal concentration/dose and also monitor the resistance to these insecticides in leafhoppers collected from different localities. Ladybird beetle C sexmaculata shows a type II functional response to increasing densities of prey i.e., Aphis gossypii at cumulative exposure of 12 and 24 hrs to it’s prey. The attack rate or searching efficiency was found to be higher at 12 hrs (0.0391/12 hrs) than at 24 hrs (0.0254/24 hrs) of exposure as against it at 12 hrs handling time was found to be shorter (0.3654 hrs or 21.92 min) than 24 hrs(0.4441 hrs or 26.65 min). Imidacloprid was the more toxic insecticide to the leafhopper with lowest LC50 value in all locations, Kanshivni (175.51 ppm), Chandur (95.92 ppm) and susceptible (21.68 ppm) as compared to acephate with LC50 values at Kanshivni (607.76 ppm), Chandur (483.37 ppm) and susceptible (51.11 ppm) Relative Toxicity of imidacloprid was higher as compared to acephate at all locations. Imidacloprid was 5.04, 3.46 and 2.36 times more toxic than acephate at Chandur, Kanshivni and in field susceptible population. Leafhopper developed 11.89 fold resistance to acephate at Kanshivni village, district Akola.The present investigation entitled “In vitro assessment of some IPM components against major sucking pests of cotton” was carried out during the year 2017-2018 in Department of Agricultural Entomology, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola. The data on mean prey consumption on all prey densities at different time periods of 3, 6, 9, 12 having equal time intervals showed that the predator feeds maximally during the first 3 hours of exposure to the prey and the amount of aphids fed decreased for the next 3 hour interval. The proportion of prey consumed also keeps on decreasing as the prey density increases for the first time period of 3 hrs and also for cumulative time periods of 6, 9, 12 and 24 hrs after exposure of the predator to the prey. The attack rate ‘a’ at a cumulative exposure of first 12 hrs was found to be higher, 0.0391/12 hrs as against to a lower attack rate at cumulative exposure of next 12 hrs i.e., 0.0254/24 hrs. However the handling time ‘Th’ was found 0.3654 hrs (21.92 min) after 12 hours of cumulative exposure which increased to 0.4441 hrs (26.65 min) after next 12 hours of cumulative exposure. This shows that the predator was more efficient as a biocontrol agent during the first 12 hrs of exposure to the prey than after that time period. In the field population of A. biguttula biguttula collected from the village of Chandur, district Akola, the LC50 of imidacloprid and acephate was worked out as 95.92 ppm and 483.37 ppm respectively. Similarly from Kanshivni village, district Akola the LC50 of imidacloprid and acephate was worked out to be 175.51 ppm and 607.76. However, in the field susceptible population of leafhopper LC50 value of imidacloprid and acephate was 21.68 ppm and 51.11 ppm respectively. Relative Toxicity of imidacloprid was higher as compared to acephate at all locations. Imidacloprid was 5.04, 3.46 and 2.36 times more toxic than acephate at Chandur, Kanshivni and in field susceptible population. Resistance to acephate and imidacloprid was observed at both the locations with resistance fold being greater for acephate. Relative Resistance to imidacloprid was 4.42 fold and 8.10 fold at Chandur and Kanshivni respectively. The Relative Resistance to acephate was 9.46 fold and 11.89 respectively.enPlant Protection, Biological control agents, Natural Enemies, Chemical Control, Toxicology, Toxicity, Pesticides, Insecticides, Crops, Field Crops , Cotton, Gossypium hirsutum, Bt Cotton, Functional response, Cotton aphid, Aphis gossypii, Ladybird beetle, Cheilomenes sexmaculata, Coccinellidae, Handling time, Attack rate, Prey density, Food exploitation, Amrasca biguttula biguttula (Ishida), Cotton leafhopper, Imidacloprid insecticide, Acephate insecticide, Relative Toxicity, Insecticidal resistance,Thesis