S.I. YUSUFZAICHOPARA KRISHNA KARSHANBHAI2021-07-172021-07-172020-06https://krishikosh.egranth.ac.in/handle/1/5810170628he study was conducted to isolate and identify probiotic bacteria from the gut of Xiphophorous helleri and Betta splendens. For isolation of gut microbes five fishes of each variety were collected. Eleven bacterial strains were isolated from the freshwater ornamental fishes. The eleven group represented by isolates of C1, C2, C4, C9, C11, C13(a), C13(b), C14, C19, FSB and FSS were selected for biochemical tests. Bacteria isolated from the gut of Xiphophorous helleri were identified as Enterobacter, Enterococcus faecalis and Bacillus subtilis by observing their colony morphology, physiological and as well as some biochemical characteristics. Microscopically they were Gram-negetive, irregular or round shaped, non- motile and few bacterial strains were catalase negative. Bacillus cereus was isolated from Betta splendens. It was Gram-positive and catalase-positive. From this study, it was concluded that the eleven bacteria isolated from intestine could be potentially developed for their use as probiotic bacteria in fish feeds.EnglishThesis