Bankar, P. S.Joshi, A. S.2017-06-142017-06-142016http://krishikosh.egranth.ac.in/handle/1/5810020186The present research work was conducted to study the polymorphism of -Lactalbumin and -Lactoglobulin genes (whey protein genes) in Marathwadi buffaloes using PCR-RFLP technique. The experiment material was comprised of (n=30) blood samples and (n=9) milk samples of Marathwadi buffaloes, collected from the Marathwadi Buffalo Farm, College of Veterinary and Animal Sciences, Udgir during June-August, 2016. The research work was conducted at Molecular Genetics Laboratory of Department of Animal Genetics and Breeding, Post Graduate Institute of Veterinary and Animal Sciences, Akola. DNA was isolated from blood using standard Phenol-Chloroform extraction method followed by checking of its quality and quantity by Spectrophotometer and subsequently by electrophoresis method. A 309 bp fragment of -Lactalbumin gene amplified using forward (5’ TTG GTT TTA CTG GCC TCT CTT GTC ATC 3’) and reverse (5’ TGA ATT ATG GGA CAA AGC AAA ATA GCA G 3’) primer with MspI restriction enzyme yielded only one type of restriction pattern (220 bp and 89 bp) in all 30 Marathwadi buffaloes and accordingly monomorphic genotype was identified as BB. A 247 bp fragment of -Lactoglobulin (LGB) gene amplified using forward (5’ TGT GCT GGA CAC CGA CTA CAA AAA 3’) and reverse (5’ GCT CCC GGT ATA TGA CCA CCC TCT 3’) primer with HaeIII restriction enzyme yielded only one type of restriction pattern (148, 99 and 74 bp) in all 30 Marathwadi buffaloes and accordingly monomorphic genotype was identified as AB. The estimates of protein, fat and lactose contents were recorded thrice at monthly interval from 9 unrelated lactating buffaloes belonging to Marathwadi Buffalo Farm, College of Veterinary and Animal Sciences, Udgir using Lactoscan (Milk analyzer) during June to August 2016. The mean values for protein, fat and Lactose content in Marathwadi buffalo ranged from 3.8 percent to 4.3 percent, 3.9 percent to 5.8 percent and 3.7 percent to 4.2 percent respectively. The total yields for protein, fat and Lactose content in Marathwadi buffalo estimated by Test Interval Method ranged from 19.66 kg to 30.52 kg, 21.99 kg to 31.11 kg and 18.68 kg to 28.65 kg, respectively. The monomorphic pattern revealed by screening of -Lactalbumin gene (BB) and -Lactoglobulin gene (AB) in small population of Marathwadi buffaloes pointed out the need of genotyping whole or larger population of ‘true to breed type’ Marathwadi buffaloes from its breeding tract so as to identify possible genotypes of whey protein genes. The monomorphic genotype AB observed for -LG gene in Marathwadi buffaloes is suggestive of presence of AA and BB genotypes in population of Marathwadi buffaloes. Screening larger population of Marathwadi buffaloes for finding polymorphism of candidate milk genes like whey protein genes is prerequisite to explore their association with economically important milk production traits will provide a robust platform for marker assisted selection.enThesis