Dr. Hiron M. HarshanARYA KRISHNAN S2024-08-122024-08-122023-08-10https://krishikosh.egranth.ac.in/handle/1/5810213122A study was undertaken to evaluate the efficacy of different therapeutic protocols in the treatment of canine pyometra based on clinical, haemato biochemical, B-mode ultrasonographic and Doppler ultrasonographic evaluation. Twenty two bitches presented to TVCC, Mannuthy and UVH, Kokkalai and confirmed with pyometra were randomly grouped into three based on the treatment followed, as group I (mifepristone @ 5 mg/kg b.wt. PO b.i.d., misoprostol @ 10 mcg/kg b.wt. PO b.i.d.), group II (mifepristone @ 5 mg/ kg b.wt b.i.d., misoprostol @ 3mcg/kg b.wt. intravaginally b.i.d.), group III (mifepristone @ 5 mg/kg b.wt. PO b.i.d.). In bitches with serum progesterone > 2ng/mL on the day of presentation cabergoline was administered @ 5 mg/kg b.wt. PO o.d. for nine days. Haematology recorded anaemia, leucocytosis with neutrophilia in patients with pyometra, which resolved after treatment. Bitches treated withmifepristone and misoprostol (oral/ vaginal) attained a faster return to normal leucogram. Serological analysis revealed elevated progesterone, alkaline phosphatase and total protein, which reduced to physiological values by day 15 in animals of all the treatment groups. No abnormal values were marked in alanine transaminase, blood urea nitrogen or creatinine in all the bitches during the duration of treatment, probably as a result of excluding pyometra affected cases from medical management, which had gross systemic affections. B-mode ultrasonography revealed distended uterine horns with anechoic to hypoechoic contents and thickened wall in all bitches, which had reduced at statistically similar rate to normal by day 15 of treatment. Bacteriological isolates from anterior vagina revealed Gram negative bacilli followed by Gram positive cocci and Gram negative coccobacilli. Maximum sensitivity was noticed to Gentamicin, followed by cotrimoxazole and Ceftriaxone tazobactum in antibiogram. Resistive index was found to be a reliable tool as a recovery marker, as they increased from values below the marker values of 0.72 to above as the treatment progressed. RI values increased to 0.72 by day 3 in bitches treated with intravaginal misoprostol. Though bitches in all the groups attained recovery, in terms of haemato-biochemical and ultrasonographic observations a faster recovery rate was recorded in bitches treated with mifepristone and misoprostol (oral or intra vgainal). Based on clinical improvement, the bitches in group II had a mean recovery time of 2.42 + 0.48 days, which was significantly lower than that of bitches in group I and III. It could be concluded that all three protocols were effective for medical management of pyometra in bitches. The combination of oral misoprostol and intravaginal misoprostol along with supportive therapy was better in terms of clinical recovery.EnglishThesis