Goel, ReetaJoshi, Divya2019-09-122019-09-122019-05http://krishikosh.egranth.ac.in/handle/1/5810127458Two psychrotolerant diazotrophs, Pseudomonas jesenii MP1 and Rhodococcus qingshengii S10107, were retrieved from department culture collection. They were tested for their impact on germination efficacy of seeds of three crops viz. chickpea, lentil and pea. Based on it, field trial experimentation was conducted on Cicer arietinum L. var. PG-186 at Norman E. Borlaug Crop Research Centre (NEBCRC), GBPUA&T to check its growth and development as well as soil health. Plant growth promotory activity of these strains were documented through increased plant growth in terms of shoot and root length, fresh and dry weight, nodule number, nodule fresh and dry weight (agronomical parameters), chlorophyll content and leaf nitrate reductase activity (biochemical parameters), nitrogen uptake, seed protein content and yield of the plants over control. Soil health was measured in terms of increased macro- and micronutrient content and soil enzyme activities unlikely to control. The results have shown that Pseudomonas jesenii MP1 with recommended dose of fertilizer (20 kg N ha-1) gave highest agronomical parameters, chlorophyll content, nitrogen content in plant, seed and straw, crop yield and harvest index over control treatment. However, MP1 alone gave highest nodule parameters, leaf nitrate reductase activity as well as bacterial (16S rRNA) and diazotrophic (nifH) copy number in soil. DGGE analysis showed that both diazotrophs remained persistent till 75 DAS. High throughput sequencing revealed soil with S10107 was characterized by the highest biodiversity indexes than control. Further, differential proteomics studies conducted on S10107 exhibit expression of proteins related to nitrogen fixation and stress response under N2-deficient conditions. Protein-protein interaction (PPI) showed interaction of major proteins as inevitable multifunctional nodes, maintaining the integrity of PPI’s network as their deletion could collapse the complete network into small clusters.ennullThesis