Tharanya, SSarathchandra, GhadevaruDhinakar Raj, GPreetha, SPTANUVAS2020-07-212020-07-212018http://krishikosh.egranth.ac.in/handle/1/5810149552TNV_TH_MVM 16051_2018This study was envisaged to screen and quantify the presence of antibiotic residues incommercial and desi chicken eggs and feed samples collected from commercial layer farm outlets in Tamil Nadu. Egg samples were separated into egg fractions (Whole egg, albumen and yolk) and extracted for five antibiotic groups (Aminoglycosides, Macrolides, Pleuromutilins, Quinolones and Tetracyclines). Extracted samples were screened by microbiological assay and confirmed and quantified by High Performance Thin Layer Chromatography (HPTLC). Microbial screening ofcommercial eggs revealed that 12.2 %, 10% and 6.1 % of contaminated samples and they were related to macrolide & pleuromutilin group, tetracycline & aminoglycoside group and quinolone group,respectively. Analysis of layer feed samples for antibiotic residues by microbial screening showed 30 % and 16.5 % samples positive to macrolide & pleuromutilin group and tetracyclines & aminoglycoside group respectively. Positive samples subjected to HPTLC had a high incidence of tiamulin residue (12.5 per cent) and the mean concentrations of residues observed in whole egg, egg white and egg yolk samples were 185.72 ± 56.27, 173.91 ± 34.12 and 325.69 ± 90.19 μg/Kg respectively with an overall range of 42.89 – 507.46 μg/kg. Mean concentrations of OTC residues observed in whole egg, albumen and egg yolk samples were 13.89 ± 0.22, 47.64 ± 0.37 and 0.38 ± 0.13μg/ Kg respectively with an overall range of 12.95 – 48.46 μg/kg. Quinolones showed positive result only in whole egg with an overall range of 4.24 – 21.58 μg/kg for enrofloxacin and 6.32 – 22.4 μg/kg for ciprofloxacin. The values obtained were below the MRL and in compliance with Codex alimentarius and EU standards. None of the desi eggs samples analysed were positive for antimicrobial residues.enVeterinary ScienceThesis