MOULI KRISHNA, K(MAJOR)SRINIVAS, MVINOD KUMAR, NGEETHA REDDY, A2018-11-302018-11-302006-05http://krishikosh.egranth.ac.in/handle/1/5810084890THESESABSTRACT: The objective of the present study was to ascertain the diagnosis of early pregnancy in crossbred cows. A total of 90 parus cyclic crossbred cows under rural management conditions were utilized. 90 cows on day 4, 7 and 22-24 post insemination were divided randomly in to three groups. In group I, II and III PGF2α 150μg, 300μg and 450μg, injected intra jugularly. While in group IV, cows served as control without drug. Blood samples were collected from every cow just before PGF2α administration. The response of PGIMFT on day 4, 7 and 22-24 post insemination was 95.00 (19/20), 95.00(19/20) and 88.23% (15/17) in group I; 100 (20/20), 100(20/20) and 71.43% (10/14) in group II; and 92.00(23/25), 92.00(23/25) and 95.24% (20/21) in group III respectively. The time interval for PGIMFT response was 163.06±16.46, 173.74±21.51 and 175.93±16.20 seconds in group I; 183.05±12.20, 198.15±17.32 and 149.4±13.43 seconds in group II and 158.75±14.20, 170.56±17.90 and 183.85±13.92 seconds in group III respectively. The serum progesterone concentration in group I on day 4, 7 and 22-24 post insemination in PGIMFT tested crossbred cows was 1.98±0.33, 0.5ng/ml; 3.48±1.07, 0.5ng/ml and 5.10±1.30, 3.0±2.25ng/ml in positive and negative cases. In group II 2.42±0.41, 0.0ng/ml; 3.29±0.73, 0.0ng/ml and 9.00±2.46 and 1.6±0.8ng/ml in positive and negative cases. In group III 2.43±0.51, 0.75±0.0ng/ml; 3.27±0.86, 3.88±3.62ng/ml and 4.81±0.98 and 2.5±0.00ng/ml in positive and negative cases, respectively. The sensitivity and specificity of PGIMFT in confirmation with serum progesterone concentration was 90.9 and 16.66% in group I; 87.5 and 50.00% in group II; 100 and 11.11% in group III, respectively and in confirmation with rectal examination was 84.62 and 0.00% in group I; 75 and 33.33% in group II; 100 and 11.11% in group III, respectively. The sensitivity and specificity of serum progesterone in confirmation with rectal examination was 76.92 and 75% in group I; 87.5% and 83.33% in group II; and 83.33 and 77.77% in group III, respectively.ennullThesis