Arora, N. K.Solanki, Shivendu Pratap Singh2023-06-292023-06-292022Solanki, Shivendu Pratap Singh (2022). Morphological and Molecular characterization of coloured genotypes of guava for yield and quality traits (Unpublished Ph.D. Dissertation). Punjab Agricultural University, Ludhiana, Punjab, India.https://krishikosh.egranth.ac.in/handle/1/5810197828The investigations on morphological and molecular characterization of coloured genotypes of guava for yield and quality traits was carried out during year 2018-19 and 2019-20 for phenotypic and genotypic characterization of coloured guava genotypes, development and validation of new F1 hybrids based on morphological and molecular characterization and linkage map construction of F2 population of Punjab Kiran variety. The results revealed that highest tree height (5.77 m) was recorded in genotype AC 8-6 whereas lowest tree height was found in CISH G-5 (4.45m), maximum tree spread (2.61m) was recorded in genotype AC 6-2. The minimum tree spread was found in AC 8-6 (2.13 m) and the highest tree volume was recorded (79.21 m3) was recorded in genotype AC 5-7. The minimum tree volume was found in AC 1-4 (45.67 m3), maximum fruit weight (211.33g) was recorded in genotype AC 8-6, Maximum thickness of outer flesh (1.77mm) was recorded in genotype AC 5-7, minimum thickness of outer flesh whereas AC 6-4 (1.00mm). maximum Core diameter (211.33g) was recorded in genotype AC 8-6 whereas minimum Core diameter was recorded in AC 5-7 (3.93mm), coloured guava genotypes showed higher range of variation in skin colour, the maximum “L” value was recorded in genotype AC 8-6 (66.71) and minimum was recorded in AC 5-2 (60.22). Maximum “a” value recorded in AC 5-7(7.11) and minimum recorded in AC 10-5 (3.29). Maximum “b” value recorded in AC 8-6 (26.64) and minimum recorded in AC 5-7 (21.69). The highest yield (43.17 kg/tree) was recorded in genotype AC 6-2 whereas lowest yield (Kg/tree) was found in AC 5-2 (39.67 kg/tree).The genotypes were grouped into two type based on their shape, they were round and oblong in shape. The highest value of TSS (10.73 %) was recorded in genotype CISH G-5, whereas, the lowest TSS (8.33%) was found AC 5-2, The titrable acidity (0.37 %) was recorded minimum in AC 5-2. Maximum titrable acidity (0.62%) in AC 10-5., The maximum Vitamin C content (123.37 mg/100 g of pulp) was recorded in genotype AC 8-6. And the minimum Vitamin C content was found AC 10-5 (98.45 mg/100 g of pulp, maximum total Sugar (6.94) was recorded in genotype CISH G-5. Minimum total Sugar was found in AC 10-5 (4.163), The number of seeds per fruit varied between 143.67 to 213.00 number of fruits in coloured guava genotypes. 100 seed weight ranged from 0.94 to 1.67 and recorded maximum in AC 8-5 and lowest in AC 10-7. The individual fruit seed weight ranged from 1.91 to 3.41 g and maximum in AC 8-5 and found lowest in AC 10-7, Seed weight per 100 g of pulp varied between 0.50 to 0.89 Seed weight per 100 g of pulp in coloured guava genotypes, High PCV value for the all traits as compare to ECV and GCV represents variability less affected by environmental factors. Estimate of heritability (h2) ranges from 0.54- 0.95, Highest positive correlation was observed with core diameter and fruit weight among the selected genotypes, path coefficient analysis (genotypic & phenotypic correlation) vitamin C content, total sugar, seed characters. Directly or indirectly all other characters are showed positive or negative effects toward yield. In cluster analysis and Mahalnobis Euclidean distance by Tocher method 10 genotypes were classified in 4 clusters. Characterization of genotypes was done using KASP markers based on marker data, coloured genotypes of guava grouped in 3 clusters .True F1 were identified using KASP marker. F2 progeny individuals of hybrid, Punjab Kiran using polymorphic markers between G-1 and 17-16 and generated three linkage group plotted on 3 chromosomes.EnglishThesis