Bunkar, Durga ShankarYogita2022-03-092022-03-092021Berberis aristatahttps://krishikosh.egranth.ac.in/handle/1/5810183229The present study entitled, “Extraction of Berberine from Berberis spp using different method and concentration” was undertaken in the laboratory of Department of Dairy Science and Food Technology with the aim of extraction using different extraction techniques from Berberis aristata and B. lycium. Berberis is a promising source of various secondary metabolites including benzylisoquinoline (berberine), natural alkaloid which is a principle active compound of this plant. Other active compounds are berbamine, oxyberberine, aromoline, oxyacanthine, palmatine. It is being used as general tonic as well as a cure for morbidity arising from different kinds of diseases. It is used in the treatment of diabetes, jaundice, malarial fever, provide relief in eye and ear infection, wound healing, gastrointestinal disorders, effective in rheumatism, analgesic action against various skin diseases, cancer, diuretic, stomachic, and anti-convulsive and also as a stimulant. Chemical characterization of isolated extract from Berberis aristata and Berberis lycium included proximate analysis covering estimation of moisture, ash, fat content, protein, phenolic content and carbohydrate. The quantitative analysis of berberine in extract was done using HPLC. The samples for quantitative analysis from roots of B. aristata and B. lycium from Solan district of Himachal Pradesh. From the present study, it can be inferred that the antioxidant activity of the extract of roots of Berberis aristata and Berberis lycium which was determined using DPPH (2, 2-diphenyl-1-picrylhydrazyl) assay exhibited dose-dependent radical scavenging activity. The proximate analysis has shown that moisture and ash content are high in B. aristata as compared to B. lycium. While, the proportions of Protein, Fat, Crude Fibre and Total Carbohydrates are present in high amounts in B. lycium as compared to B. aristata. In order to develop and validate an efficient method for the analysis of berberine in extracts of different solvents and concentration were explored. According to the past studies the detection wavelength used is 346 nm and the mobile phase of acetonitrile/buffer used in gradient flow. Before fully implementing in the quantitative determination of berberine, this method was thoroughly validated for its linearity, specificity, accuracy, precision and intermediate precision, and robustness under various modified conditions.EnglishThesis