Jayashankar, M.R.Nagaraja, R.Bhaskaran, R.Girish Kumar, V.Umesha Kavali2016-11-142016-11-1412-12-14http://krishikosh.egranth.ac.in/handle/1/85458Pg. ThesisA study was conducted with the objective of analyzing the genetic variation at Alpha S1 Casein gene region in Nandidurga non-descriptive goats of Karnataka by using PCR - RFLP technique. Genomic DNA was isolated from blood samples of 85 animals. The genetic diversity of caprine Alpha S1CSN locus was investigated by PCR - RFLP by amplifying part of intron 8, exon 9 and part of intron 9 of the Alpha S1 CSN gene. Restriction endonuclease enzyme XmnI was used to detect the genetic variation of the experimental unit in Alpha S1 CSN gene. Upon PCR - RFLP analysis, two patterns were observed which allowed the identification of two alleles A and B, and two genotypes, AA and BB. Further PCR - RFLP study in 35 Osmanabadi and 50 Bidri goats prevalent in North Karnataka revealed similar observations. Ironically AB type was not observed in any of these goats. The sequence analysis indicated that there was a high homology between the present result and the published caprine Alpha S1CSN gene sequences. There was query coverage to the extent of 100 per cent. This is one of the first studies in non-descript goats. Presence of predominant 'A' allele among these goats provides other selection criteria in addition to traditional selection methods, for increased milk protein.enStudies on Genetic Polymorphism of Alpha S1 Casein Gene in Nandidurga GoatsThesis