Z. A. HaiderShweta Singh2024-10-072024-10-072006https://krishikosh.egranth.ac.in/handle/1/5810215424Celastrus paniculatus wild, a member of family celastraceac is a perennial climber which is now enlisted as an endangered plant due to its prolong seed dormancy, poor germination and viability. Also indiscriminate over exploitation of this plant by the pharmaceutical industries have led it to the verge of extinction. It is used to improve intelligence quotient to cure leucoderma headache and gout etc. Through the present study effort has been made to develop a protocol for mass multiplication of this medicinally important plant so as to bring it out of red zone. Surface sterilization of the leaves and axillary buds had been standardized using 0,05% Hgcl2 for 10 Minutes. These decontaminated explants were inoculated on Murashige and Skoog”s (1962) medium supplemented with different growth regulators for shoot multiplication and callus formation MS medium with 1.0 mg/1BAP, 0.1mg/1NAA, 50mg/I ascorbic acid and 25mg/l Adso4 produced a maximum average numbers of shoots after two month of inoculation with subcultures at every 15 days interval Large sized calli were obtained on M.S medium supplemented with 1.0mg/l of each NAA and Kn. Colchicum luteum Baker is herbaceous plant belonging to family liliaceae. Its FDA approved medicinal use is in the treatment of gout. It has also been beneficial in the treatment of rheumatic swelling contents of colchicine in C, luteum is comparable to those present in C autumnal a potent source of colchicine for scientific and medicinal use C luteum is also enlisted as threatened plant. Through this study, a viable protocol for in –vitro micro propagation using corms as explants has been standardized. Surface sterilization of the explants of C. luteum was standardized with 0.15% (W/V) Hgcl2 F for 20 minutes. The surface sterilized explants when inoculation on M.S. Medium with 5.0mg/1 BAP,50.0mg/I AdSO4 and 10.0mg/ I citric acid showed maximum average shoot multiplication of 9.57 after 60 days of inoculation. Excised shoot lets from those multiplied earlier produced a maximum average of 6.67 rootlets on M.S medium with 0.5mg/IBA. These rooted plantlets were subjected to primary and secondary hardening , 70% plants survived the acclimatization. Preliminary DNA study throught RAPD between mother plant and tissue culture hardened plant of c. luteum on M.S. Medium supplemented with 5.0mg/I BAP, 50.0mg/l Adso4 and 10.0mg/l citric acid Reflected some differences at genic level. Also, the same study between tissue cultured shootlets on MS medium supplemented with 1.0 mg/I BAP, 0.1mg/1 NAA,Adso4 and leaf callus grown on MS medium supplemented with 1.0mg/l NAA and Kn each showed some variations at genic level but the RAPD studies need to be replicated for coming to any exacr conclusion.EnglishThesis