Jain, R.K.Virender Kumar2016-10-252016-10-252016http://krishikosh.egranth.ac.in/handle/1/81884Present investigation was carried out to develop efficient micropropagation protocol(s) using shoot tip and nodal explants in two cultivars of guava (Psidium guajava L.), Hisar Safeda and Hisar Surkha. Experiments were conducted to carry out surface sterilization of explants from field grown plants and study the effect of different concentrations and combinations of growth regulators on in vitro shoot induction, multiplication and rooting. Explants were best surface sterilized with 0.2% CaOCl2 for 10 min followed by 0.1% HgCl2 for 5 min which resulted in >40% survival of the explants. Explants showed maximum shoot induction (>47%) in MS medium supplemented with BAP 5 mg/L alone in combination with NAA 0.1 mg/L. The primary proliferated cultures were transferred on to various MS media supplemented with different concentration and combination of growth regulators for shoot multiplication. MS medium supplemented with BAP 5.0 mg/L, BAP 5.0 mg/L + NAA 0.1 mg/L, Kinetin 5.0 mg/L, Kinetin 5.0 mg/L + NAA 0.1 mg/L, BAP 2.5 mg/L + Kinetin 1.0 mg/L and BAP 5.0 mg/L + Kin 1 mg/L supported shoot multiplication (>2.0 shoots per culture) both from nodal and shoot tip explants after 30 days. Multiple shoots were surgically separated and cultured on to various MS based rooting media; frequency of root induction was maximum (>62%) on half MS medium containing IBA (1.0 mg/L) in both cultivars. In vitro raised plants were transplanted and hardened in pots containing sterile 3:1soil and sand mixture. The survival percentage of plants in pots was 50%.enPlanting, Guavas, Biological phenomena, Cytokinins, Vegetative propagation, Auxins, Biological development, Tissue culture, Fruits, IbaThesis