KANWAR, KAMLESHRANA, SABINA2016-11-152016-11-152013http://krishikosh.egranth.ac.in/handle/1/85678ABSTRACT The present investigation “Assessment of genetic diversity in Aloe vera L. among different provinces of H.P.” was undertaken using morphological, biochemical, Random Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeats (ISSR) markers. On the basis of morphological and biochemical characters very low variation was found among selected genotypes. A total of 30 RAPD primers and 6 ISSR primers were screened and out of 30 only 24 RAPD and all the 6 ISSR primers gave amplification. In RAPD analyses, a total of 91 bands were amplified, out of which 82 were polymorphic in nature however in case of ISSR markers, 21 polymorphic bands were observed from a total of 24 bands. The amplified products from both the analysis ranged from 100 to 3000 bp and 250 to 1500 bp respectively. Maximum number of bands 107 was scored with primer OPE-10 during RAPD analysis and 114 bands with primer hb-13 during ISSR studies. The similarity coefficient value ranged from 0.62 to 0.91 on the basis of dendrogram. Cluster analysis, using UPGMA, SAHN clustering (NTSYS-pc ver. 2.0) grouped the genotypes into 4 main clusters. C1 genotype showed its distinct nature by sidelining at 0.62 similarity index value leaving behind 65-91% genetic similarity among the remaining genotypes. On the basis of dendrogram formed by ISSR markers 57-100% of genetic similarity was obtained forming two clusters representing 3 and 21 genotypes in each, thereby depicting 80% similarity in minor and 65% similarity in major cluster. Hence the study showed existence of genetic relatedness within the species.enAloe vera L,genetic diversityThesis