Gupta, NamartaGoyal, Priyanka2019-12-142019-12-142019http://krishikosh.egranth.ac.in/handle/1/5810137689In the present investigation, the influence of salt stress on physiological, biochemical and molecular parameters in barley (Hordeum vulgare L.) genotypes at different salinity levels was studied. Seeds of ten barley genotypes (BL1400, BL1314, PL890, PL807, RD2552, PL751, BL1430, PL426, BL1375 and BL1367) were treated with different salt concentrations (0, 25, 50, 75, 100, 125, 150, 175 and 200 mM NaCl). The salinity stress has deleterious effect on seed germination and seed vigour parameters. Increased salinity caused a significant reduction in percentage of germination and seed germination rates. Also, a significant reduction in root and shoot length of seedlings was recorded. Seedling biomass (fresh and dry weight) declined in stressed seedlings as compared to control. Among all salt concentrations effect of salt stress was more differentiable at 75mM and 100mM. Therefore, these salinity levels were selected to test the effect of salt stress on biochemical parameters. All biochemical parameters viz. total soluble sugar content, total soluble protein content and activity of antioxidant enzymes (catalase, peroxidase and superoxide dismutase) increased with increased salt stress. Also, molecular studies were conducted on comparatively sensitive and comparatively tolerant genotype under selected salt concentration levels.ennullThesis