Kamdi, B. P.Kudale Atul Pandurang2024-11-042024-11-042024-03-02https://krishikosh.egranth.ac.in/handle/1/5810216008Bovine pneumonia is a considerable ailment that profoundly affects the livestock sector and great impact on mortality of animal. The primary objective of the present investigation was to examine the occurrence of bovine pneumonia in the Akola district, along with the associated pathomorphology caused by a specific pathogen Bovine parainfluenza virus 3. Out of total 1410 animals, 1230 carcasses were inspected for any pulmonary lesion and 180 animals were screened for any respiratory signs during April 2023 to December 2023, 325 bovine carcasses showing pneumonic lesions were collected for pathological and molecular studies. Grossly, lung displayed cranio-ventral consolidation, either red or grey hepatisation with firm to rubbery consistency and exudate varied from frothy to mucupurulent in trachea, bronchi, bronchioles and alveoli. The cranioventral segment exhibited frequent consolidation in the right cranial lobe. The incidence of bovine pneumonia in Akola district was 26.42% (325/1230). On histopathological examination interstitial pneumonia (29.84%, 97/325) had more occurrence followed by bronchopneumonia (21.84%, 71/325), bronchointerstitial pneumonia (5.53%, 18/325), granulomatous pneumonia (2.46%, 8/325). As bronchopneumonia further classified as suppurative bronchopneumonia (17.53%, 57/325) and fibrinous bronchopneumonia (4.30%, 14/325). The incidence of pulmonary hydatidosis was (10.65%, 131/1230). The total 254 sample examined for bovine parainfluenza virus 3 by PCR with specific primers (328bp). The incidence of bovine parainfluenza was 5.51%. The BPIV-3 positive cases had acute bronchointerstitial pneumonia (04), suppurative bronchopneumonia (01), fibrinous bronchopneumonia (02) and interstitial pneumonia (03). On phylogenetic analysis, All four isolates of the current study were homologous to each other and closely related to the Asian BPIV-3 isolates from Japan and China and distantly related to the isolates from USA. The present study indicated 26.42% occurrence of bovine pneumonia and 5.51% incidence of bovine parainfluenza virus 3 in Akola district.EnglishThesis