Thind, K.S.Jagmeet Singh2019-09-132019-09-132019http://krishikosh.egranth.ac.in/handle/1/5810127610In the present studies, a set of one hundred twelve diverse varieties/clones including twelve checks of sugarcane were evaluated for variability, heritability, genetic advance and correlation coefficient. The aim of present study was to study genetic variability and molecular characterization for sucrose synthesis gene(s) in Sugarcane (Saccharum spp. complex). All the genotypes were planted in a augmented design. The observations on eight cane yield, its components traits and twelve quality traits were recorded. Analysis of variance revealed significant differences among clones/varieties for all the traits. For agromorphological traits, 19 new clones were superior than high yielding check variety CoPb-93.For quality traits, 8 new clones superior than quality check variety CoJ-64.Two clones K-11/9 and K-11/4 were recorded as high yield clones than high yielding check variety CoPb-93 and high quality than high quality check variety CoJ-64.Two clones K-31/2 and K-47/115 better in quality with CoJ-64 and at par the yield with CoPb-93.High magnitude of coefficient of variation i.e. PCV and GCV under cane yield and its component traits was recorded for number of shoots at 120 days and cane yield. High heritability coupled with high genetic advance recorded for number of shoots at 120 & 240 days (000/ha) and cane yield (kg/plot).For quality traits, high PCV, GCV and ECV was recorded for fibre (%). The genetic diversity in a set of 124 (112 clones and 12 check varieties) genotypes of sugarcane was assessed for sucrose phosphate synthetase gene with 10 different set of SSR markers. Different polymorphic percentage that shows high level of diversity was obtained in eight SSR markers. Marker A, SPS, SCB07, MSSCIRI and MSSCIR43 showed higher polymorphic percentage (100%).The divergence analysis grouped 124 genotypes into three major clusters. It was observed that more number of clones belongs to Cluster II. In Cluster III, eight clones and one check (CoJ-88) were present. Maximum similarity was observed in K-1/6 and K-1/30, K-3/18 and K-47/93, K-1/9 and K-48/103 .Minimum similarity was observed in K-11/9 and K-12/13, K-47/41 and CoPb-93. The varieties/clones having high yield and sugar and with high genetic diversity were identified that can be utilized for future evaluation on large scale in sugarcane breeding programme.ennullGenetic Variability and Molecular Characterization for Sucrose Synthesis Gene (s) in sugarcane (Saccharum spp. complex)Thesis