Chhabra, A.K.Dahake, Ashwin B.2017-06-032017-06-032014http://krishikosh.egranth.ac.in/handle/1/5810014899A research programme “Search for fertility restorer and sterility maintainer lines for timopheevi cytoplasm in wheat (Triticum aestivum L.)” was conducted during Rabi season of 2010-11 and 2011-12 at experimental farm area of Department of Genetics and Plant Breeding, CCS HAU, Hisar. For the present investigation, three cytoplasmic male sterile lines, viz., D 2802-3A, WH 147A and WH 147 2A carrying sterile cytoplasmic background of T. timopheevi were used as female parents to produce sixty six F1 hybrids by crossing with twenty five elite wheat cultivars in the Rabi season of 2010-11, These hybrids were grown under timely sown normal condition in Rabi season of 2011-12 and observations were recorded for traits viz., days to heading, plant height (cm), number of tillers per plant, number of florets per spike, number of grains per spike, percentage of seed-set. Twenty eight parents were also grown under timely sown normal condition in Rabi season of 2011-12 and observations were recorded for traits viz., Glumes opening angle (degree), anther size (mm2), filament length (mm), pollen viability (%), anther extrusion (%), size of lodicules (mm) and anther colour. To identify maintainers and restorers, five spikes were bagged before anthesis in five plants in each of the sixty six hybrids, from three replications. Seed-setting in these bagged spikes were used to identify maintainers and restorers. Eighty seven lines (25 pollen parents, 3 male sterile female parents and 54 F1 hybrids) were used for molecular analysis by using fourteen RAPD primers. At the end of the experiments, it was concluded that pollen parents WH 1062 and PBW 343 were partial restorer and nineteen pollen parents were maintainer for all three CMS lines. The only one pollen parent HD 3059 was found common effective maintainer for all the three male sterile lines. Analysis of variance showed presence of ample variation for all the traits under observation. Among the 28 lines, glum opening angle and size of lodicules were more in sterile line as compared to fertile lines. Anther size, anther extrusion, filament length and pollen viability were observed more in fertile lines in comparison to sterile lines. Floral traits in relation to hybrid breeding that promote outcrossing. Our results indicated that chasmogamy is present in these lines and it is essential for exploitation of hybrid wheat. The DNA were isolated from eighty seven genotypes by CTAB method in the departmental laboratory. Sixty RAPD primers were used and standard protocols were followed for DNA isolation and RAPD analysis. 14 primers were found polymorphic and primer OPA 10 was most informative RAPD marker on the basis of polymorphism information content among all the primers used for the material under studied. All the three male sterile lines showed different banding patterns for 10 primers. Floral traits in relation to hybrid breeding that promotes outcrossing was studied and molecular characterization of A/B/R lines were done to facilitate their registration in the national genetic recourses.enwheats, hybrids, cytoplasm, developmental stages, planting, genetics, fertilizers, genotypes, rapd, dnaThesis