Ram, R.N.Goswami, Kusumlata2021-07-302021-07-302021-02https://krishikosh.egranth.ac.in/handle/1/5810171147The present investigation was designed to evaluate the biosorption efficiency of leaves and bark extract of our multipurpose indigeneous trees i.e. Kafal (Myrica esculenta) as well as Banj (Quercus leucotrichophora) against copper ion adsorption. Assessment of acute and chronic toxicity of copper sulphate and the synergic effect of plant extract and heavy metal copper was checked in Amur carp for the duration of 6 months. Amur carp fingerlings were exposed for the duration of 96 hours against 7 different concentrations of copper starting from 1ppm to 4ppm with an increment of 0.5ppm i.e. 1ppm, 1.5ppm, 2ppm, 2.5ppm, 3ppm, 3.5ppm and 4ppm. The 96 hrs LC50 value of CuSO4 to Amur carp fingerlings was found to be 1.811 ppm by Finney’s probit analysis method of data evaluation for acute toxicity bioassay. The lethal toxicity test (LC50) concluded that there is direct positive relationship between the copper concentration and mortality count. During the biosorption experiment, it was observed that Kafal leaves served as best biosorbent as it can remove upto 61.8% of copper ions from the solution in 120 minutes. The per cent removal of copper by Banj leaves, Banj Bark and Kafal bark were 59.8%, 58.7% and 56.9% respectively in decreasing order. There was increasing trend of percentage removal and adsorption capacity with contact time, which may be due to availability of binding sites in biosorbent materials, but after 90 minutes it become constant due to saturation of available metal binding sites. The present study also showed that the chronic sub-lethal copper concentration has a profound effect on Amur carp health, which is represented by noticeable changes in its behavior, growth parameters, haematological parameters and serum biochemical parameters. However, the use of biosorbents which are prepared from our indigenous tree resources can partially reduce the ill effects of copper toxicity against Amur carp upto a certain extent.EnglishThesis