D. S., AMBIKAKHICHADI, TEJASHWINI NEELAKANTHM. P., BASAVARAJAPPAD. L., RUDRESHEVOOR, SHASHIKANTH2020-09-242020-09-242019-08UHS17PGM908https://krishikosh.egranth.ac.in/handle/1/5810151886Cucumber mosaic virus (CMV) is the most destructive disease of cucumber. Survey conducted in six taluks of Bagalkot district during 2018-19 revealed that, disease incidence ranged between 13.33 to 66.66 per cent. The highest disease incidence was observed in Badami taluk (49.58%) and lowest was in Jamkhandi taluk (29.50%). The virus was transmitted mechanically and by aphids on cucumber. Different symptoms of CMV viz., small greenish mosaic patches on young leaves, dark green mottling, down ward curling of leaves and stunting of plants was observed. The indexing of host plants has shown symptoms viz., chlorotic local lesions and mosaic on Datura stramonium, D. metel. Leaf distortion and mosaic symptoms were appeared in Capsicum annuum cv. California Wonder. Whereas, Nicotiana tabacum var. White Burley, N. tabacum var. Samsun shown systemic mosaic and N. glutinosa shown mottling of young leaves. Necrotic local lesions were observed on Phaseolus vulgaris and Vigna unguiculata cv C152. In Chenopodium amaranticolor and C. quinoa chlorotic local lesions were observed, and in case of Cucumis sativus severe mosaic symptoms was seen. Aphis gossypii required minimum of 20 and 30 min acquisition access period for transmission of 100 per cent CMV. Aphis gossypii with 30 min of acquisition and one hour of inoculation feeding period was enough for 100 per cent transmission of CMV. When 10 aphids per plant were used, it can able to transmit 100 per cent of CMV. Virus reacted strongly to CMV specific antisera through DAS-ELISA. PCR product of CMV with coat protein (CP) primers indicated 163 bp band, which confirm the CMV virus. CMV in cucumber was effectively managed by seed treatment with imidacloprid 600FS @ 9 ml/kg followed by spray with acetamiprid @ 0.2 g/lit and fipronil @ 1 ml/lit at 15 days intervals which recorded the lowest mean vector population, per cent incidence, high vine length and higher yield compared to control.EnglishThesis