NUTHAN, LREDDY LAKSHMI, V2019-02-232019-02-232007-09-22Th-8927http://krishikosh.egranth.ac.in/handle/1/5810096703Present investigation on "stsmdaidization of hardening protocol of tissue cultured mulberiy plants {Moms spp.)" was carried out to standardize the hardening protocol for mulberry genotj^es through nodal explants using five genotypes viz., Vi, S36, (Irrigated); S13, RFS135 (Rainfed) and Mysore local (Control). Nodal explants of all the genotypes exhibited survival of 79.36 per cent when treated with 0,1 per cent HgCb for about 8 minutes. S36 recorded maximum callus initiation (60.76%) compared to the other genotypes, when treated with 2.0mg/L 2, 4-D along with 0.75 or l.OOmg/L BAP, 2, 4-D therefore it was identified as the best auxin for callus induction. Callus differentiation into shoots was also maximum in S36 variety, when MS medium used was fortified vdth combination of BAP (2mg/L) and NAA (O.lmg/L). Significantly higher rooting percentage (48.07%, 40.82%) was recorded when shoots were transferred to MS media having 1.0 mg/L and 1.5 mg/L NAA respectively as compared to control (22.50 %). Maximum number of leaves were recorded when 50 per cent and 75 per cent glycerol were applied as antitransparents (4.40. 4.13), followed by low melting paraffin wax (4.00) and 50 per cent grease petroleum (3.93) compared to control (2.93). Shoot length recorded maximum when grown on potting mixture (Peat: perlite: vermiculite (1:1:1)+ MS+1 ppm of NAA+lppm IBA +lppm IAA+(5.46) while sand: soil: perlite fl/2 MS+lppm lAA+lppm IBA+lppmlAA stood next (5.40). Maximum plant height was recorded significantly with the 50 per cent shading (3.69%) and 75 per cent shading (3.42%). Survival rate was recorded significantly higher in mulbeny with 45 days (81.94%) and 30 days after planting (80.89%). Considering callus initiation, shoot, internodal length, root formation, plant height, number of leaves and survival per cent, genot3^e S36 performed best out of all other varieties tried.ennullThesis