Narayan Bhatt, M.Yathiraj, S.Ramesh, P.T.Rathnamma, D.Shrikrishna, IsloorNagendra R. HegadeJouher Sulthana, C.M.2016-07-222016-07-222014-08-21http://krishikosh.egranth.ac.in/handle/1/69341Ph.D. ThesisA total of 296 quarter milk samples were collected from 74 buffaloes of different age groups, parity and stage of lactation from two adjoining districts Tumkur and Chitradurga of Karnataka state. Data regarding the pattern of milking and type of flooring of the shed was also collected during sampling. Overall occurrence of bubaline clinical and subclinical mastitis was 10.13% and 63.15% respectively. Occurrence of clinical mastitis was higher in age group of 9-11 years whereas, occurrence of SCM was higher in 3-5 year age group. Occurrence of bubaline mastitis was higher in higher parity buffaloes. Occurrence of bubaline mastitis was higher in early lactation. Occurrence of bubaline mastitis was higher in hindquarters but there was no significant difference in occurrence when compared to quarters. Bubaline mastitis was higher in buffaloes which are housed in kaccha (soil) flooring and milked by knuckling method. In the present study high correlation coefficient was seen between SCC and NAGase activity and low correlation coefficient was seen between BTB with EC and CMT. CMT was highly sensitive and NAGase activity measurement was highly specific test. EC and BTB may not be useful as a single diagnostic technique. There was increase in protein% and decrease in fat% and SNF% in SCM milk samples. Coagulase-negative Staphylococci were the most prevalent organisms isolated from subclinical mastitis. Bacterial isolates were highly sensitive to Chloramphenicol and least sensitive to Penicillin-G. SCM cases need to be treated with vitamin E and selenium and trisodium citrate to prevent infection. The supplementation of vitamin E and selenium or trisodium citrate were found to be more effective, economical and safe for animals. Further, milk was also safe for human consumption.ensowing, biological development, planting, yields, auxins, inorganic acid salts, pigeonpeas, developmental stages, drying, areaThesis