Vashishat, NishaNimrat Kaur2022-05-072022-05-072021Nimrat Kaur (2021). Ameliorative potential of Vitamin C against Bisphenol A induced alterations in reproduction of male albino rats (Unpublished M.Sc. thesis). Punjab Agricultural University, Ludhiana, Punjab, India.https://krishikosh.egranth.ac.in/handle/1/5810184447Bisphenol A (BPA), has been overused worldwide and acts as an endocrine disruptor generating genotoxic and cytotoxic effects in male reproductive system by disturbing cellular antioxidant balance causing oxidative stress. Hence, this study focussed on amelioration of BPA induced toxicity by supplementing Vitamin C. Rats were treated for 45 days with 20 mg/kg b.w. BPA, 20 mg/kg b.w. BPA+40, 80, 120 and 160 mg/kg b.w. Vitamin C. BPA gavaged rats showed hyperactivity, irritability, wounds and weakness and no such symptoms were seen in other groups. BPA significantly decreased body weight and organ weight of reproductive organs as compared to control but were significantly increased in Vitamin C groups. Histological alterations like tubular distortions and widening of interstitium were observed in testis and cauda epididymis of rats treated with BPA alone. Sperm motility, viability and count significantly decreased in BPA group with increase in sperm abnormalities whereas Vitamin C reversed BPA caused changes. BPA significantly decreased plasma levels of FSH, LH and Testosterone and increased Estrogen while Vitamin C significantly increased FSH, LH and Testosterone and significantly decreased Estrogen. SOD, CAT, GR, GPx, GST, total lipids and phospholipids decreased in testis and epididymis of BPA rats and increased at different doses of Vitamin C. BPA significantly increased LPO and cholesterol but Vitamin C decreased them in dose dependent manner.EnglishThesis