Jayachandran Nair, C SHutke Vikram, RajendraKAU2019-06-302019-06-302012http://krishikosh.egranth.ac.in/handle/1/5810110622PGFor lateral bud explants running tap water wash for 10 min. and for apical bud explants rinsing in water with wetting agent for 30 min. were effective. For removal of fungal contamination 1.0% bavistin for 60 min. was found to be the best treatment. For removal of bacterial contamination treatment with chloramphenicol, streptomycin and cephalexin each 250 mg -1l for 24 hours was the best. For lateral bud explants use of mercuric chloride 0.1% for 10 min. and for apical bud explants 0.08% mercuric chloride for 10 min. was the best. In case of survival percentage full strength MS medium and full strength MS medium + 0.1% activated charcoal equally recorded the highest survival percentage. Highest number of cultures showing bud initiation in culture establishment media was observed in full strength MS medium and full strength MS medium + 0.1% activated charcoal. The number of buds initiated per culture was the highest in full strength MS medium + 0.1 % activated charcoal. The number of days taken for bud initiation from lateral bud explants of variety Pusa Nanha was the lowest in full strength MS medium + 0.1 % activated charcoal while for apical bud explants full strength MS medium was the best. For variety Pusa Dwarf using lateral bud explants half strength MS medium and for apical bud explants full strength MS medium + 0.1 % activated charcoal recorded the lowest number of dsys. Survival percentage of lateral and apical bud explants of Pusa Nanha was the highest in NAA 0.5, BA 3.0 mg l -1. In Pusa Dwarf, the highest survival percentage of lateral bud explants was noted in the treatment NAA 0.5, BA 5.0 mg l -1. In apical bud explant NAA 0.5, BA 5.0 mg l -1 and NAA 1.0, BA 3.0 mg l -1 were effective. In lateral bud explant of Pusa Nanha NAA 0.1, Kinetin 1.0 mg l -1 showed the lowest number of days for bud initiation while in apical bud explants IAA 0.5, BA 1.0 mg l -1 registered the shortest number of days. In lateral bud explants of Pusa Dwarf IAA 0.5, Kinetin 2.0 mg l -1 resulted in lowest number of days for bud initiation while in the apical bud explant NAA 1.0, BA 3.0 mg l -1 gave the best results. The treatment NAA 0.5, BA 3.0 mg l -1 resulted in the highest number of shoots per culture from the lateral bud explants of Pusa Nanha whereas in apical bud explant NAA 0.5, Kinetin 3.0 mg l -1 was the best. NAA 0.5, BA 5.0 mg l -1 resulted in the highest number of shoot per culture in the lateral bud explants of Pusa Dwarf while in apical bud explant IAA 2.0, Kinetin 3.0 mg l -1 gave the best results. Length of shoot was the highest in Pusa Nanha lateral bud explant when BA 3.0 mg l -1 was used while in the apical bud explants NAA 0.5, Kinetin 3.0 mg l -1 was the best treatment. In case of both types of explants in Pusa Dwarf IAA 0.5, Kinetin 1.0 mg l -1 recorded the longest shoots.ennullThesis