Agrawal, U.S.Maurya, Amit Kumar2019-06-192019-06-192004-12http://krishikosh.egranth.ac.in/handle/1/5810109056Oils derived from microbial sources are termed as ‘Single Cell Oils’ (SCOs). An agro-industrial byproduct, ‘Sugarcane Molasses’ was used as the source of carbon, since it has the potential of lowering the cost of raw material. On the basis of literature reviewed two oleaginous organisms, namely, Rhodotorula glutinis var. glutinis (maximum lipid content, 70 % d.b.) and Rhodotorula minuta var. minuta (maximum lipid content, 30 % d.b.) were procured from IMTECH, Chandigarh. The average values of lipid content (LC) and total lipid produced (TL), for R. glutinis and R. minuta, were found to be 25.3418 % and 1.9292 g and 24.0263 % and 1.6457 g, respectively. At 95 % confidence level cell biomass, CONF0.95(7.3283≤8.3846≤9.4410), for R. glutinis was more than R. minuta, CONF0.95(6.6302≤6.8414≤7.0525), without any overlapping of ranges. Further, the incubation period for R. glutinis was lower than that of R. minuta by twenty four hours. Hence, R. glutinis R. glutinis was selected from the two organisms for further detailed studies. C/N ratio (23.18, 30, 40, 50 and 56.82), concentration factor (0.664, 0.8, 1, 1.2 and 1.334 l) and incubation period (58.544, 64, 72, 80 and 85.456 h) were selected as the experimental parameters. The individual optima values obtained for C/N ratio, concentration factor and incubation period were 48.5070, 0.7222 l, 67.2480 h; 50.4310, 1.0811 l, 82.5464 h and 47.4970, 0.6967 l, 69.1256 h for cell biomass (9.1296 g), lipid content (28.0566 %) and total lipid produced (2.4208 g). The recommended optimum conditions obtained for the production of Single Cell Oil were 47.4970, 0.6967 l and 69.1256 h for C/N ratio, concentration factor and incubation period and corresponding values for cell biomass, lipid content and total lipid produced were 8.9798 g, 27.1290 % and 2.4208 g. On the basis of contour graphs it was observed that C/N ratio has the most profound effect on the production of cell biomass, lipid content and total lipid produced.ennullThesis